<i>Bchex</i> virulence gene of <i>Botrytis cinerea</i>: characterization and functional analysis

Simple item page
dc.contributor.authorAguayo, C.
dc.contributor.authorRiquelme, J.
dc.contributor.authorValenzuela, P. D. T.
dc.contributor.authorHahn, M.
dc.contributor.authorSilva Moreno, E.
dc.date.accessioned2025-01-21T00:01:37Z
dc.date.available2025-01-21T00:01:37Z
dc.date.issued2011
dc.description.abstractWe previously identified Bchex as a highly expressed gene during filamentous growth in Botrytis cinerea. The gene encodes the principal protein of the Woronin body and has been shown to seal septal pores in response to cellular damage. In the present study, Southern blot analysis of genomic DNA indicated that the gene exists as a single copy in the B. cinerea genome. The gene was differentially expressed during various developmental stages: expression was high in germinating conidia and the mycelial stage and lower in resting conidia and the appressorial stage. For functional analyses, homologous recombination was used to obtain a Delta Bchex knockout mutant. Growth of the mutant was strongly reduced growth in complete medium and in defined media with sucrose, fructose or pectin as the carbon source. After detached tomato leaves were inoculated with the Bchex mutant, lesion development was markedly reduced compared to the control, suggesting that Bchex participates in normal growth, germination and virulence of this fungus.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s10327-011-0311-4
dc.identifier.eissn1610-739X
dc.identifier.issn1345-2630
dc.identifier.urihttps://doi.org/10.1007/s10327-011-0311-4
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95388
dc.identifier.wosidWOS:000292216200002
dc.issue.numero4
dc.language.isoen
dc.pagina.final238
dc.pagina.inicio230
dc.revistaJournal of general plant pathology
dc.rightsacceso restringido
dc.subjectBotrytis cinerea
dc.subjectWoronin body
dc.subjectVirulence
dc.title<i>Bchex</i> virulence gene of <i>Botrytis cinerea</i>: characterization and functional analysis
dc.typeartículo
dc.volumen77
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas