A new multiplex PCR assay for the simultaneous detection of vancomycin-resistant enterococci from rectal swabs

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dc.catalogadoraba
dc.contributor.authorBenadof, D.
dc.contributor.authorSan Martín, M.
dc.contributor.authorAguirre, J.
dc.contributor.authorParedes, L.
dc.contributor.authorMalig, R.
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorLehouque, G.
dc.date.accessioned2025-02-06T20:09:35Z
dc.date.available2025-02-06T20:09:35Z
dc.date.issued2010
dc.description.abstractObjectives This study describes the diagnostic performance of a recently available multiplex PCR-based kit for the simultaneous detection and identification of Enterococcus faecium, Enterococcus faecalis, vanA, vanB, vanC1 and vanC2/C3 genes, directly from rectal swabs constituting the most complete existing molecular assay currently available. Methods The diagnostic performance of this assay was evaluated by a multicenter study involving three independent public hospitals and consisted in the analysis of 187 rectal swabs from patients at high risk for vancomycin-resistant enterococci colonization. Results When bacteria culture was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the assay were 96.8%, 76.0%, 67.7% and 97.9%, respectively. When a composite reference standard consisting of culture and DNA sequencing of PCR products was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the PCR-based assay were 97.8%, 96.9%, 96.7% and 97.9%, respectively. Conclusions Based on these results, we conclude that this assay is considerably more sensitive than traditional microbiological methods for detecting vancomycin-resistant enterococci from rectal swabs. It is also much faster than culture. We believe that the implementation of this assay in routine clinical laboratories could help to reduce hospital-acquired vancomycin-resistant enterococci infections.
dc.format.extent6 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1016/j.jinf.2010.02.011
dc.identifier.eissn1532-2742
dc.identifier.issn0163-4453
dc.identifier.scopusid2-s2.0-77952546963
dc.identifier.urihttps://doi.org/10.1016/j.jinf.2010.02.011
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102199
dc.identifier.wosidWOS:000277203300007
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final359
dc.pagina.inicio354
dc.revistaThe Journal of infection
dc.rightsacceso restringido
dc.subjectVancomycin-resistant enterococci
dc.subjectMolecular biology assay
dc.subjectMultiplex PCR
dc.subjectIn vitro diagnostics
dc.subjectControl of hospital-acquired infections
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA new multiplex PCR assay for the simultaneous detection of vancomycin-resistant enterococci from rectal swabs
dc.typeartículo
dc.volumen60
sipa.codpersvinculados82342
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