Expert opinion on the use of platelet secretion assay for the diagnosis of inherited platelet function disorders: Communication from the ISTH SSC Subcommittee on Platelet Physiology

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dc.contributor.authorMezzano, Diego
dc.contributor.authorHarrison, Paul
dc.contributor.authorFrelinger, Andrew L., III
dc.contributor.authorMumford, Andrew D.
dc.contributor.authorNoris, Patrizia
dc.contributor.authorLordkipanidze, Marie
dc.contributor.authorGresele, Paolo
dc.date.accessioned2025-01-20T21:05:01Z
dc.date.available2025-01-20T21:05:01Z
dc.date.issued2022
dc.description.abstractAssessment of platelet secretion is crucial for diagnosing suspected inherited platelet function disorders (IPFD). A previous survey of the SSC on Platelet Physiology of the ISTH and a comprehensive review highlighted that most of the platelet secretion assays (PSAs) lack standardization and validation. The aim of this study was to provide expert consensus guidance on the use of PSAs for IPFD diagnosis. We surveyed 26 experts from 10 different countries using the RAND/UCLA methodology, to attain a consensus on sensitivity, specificity, feasibility, time to readout, and cost of most PSAs. Answers were then graded in three categories: appropriate, uncertain, and inappropriate. Equivocal or misinterpretable statements required a second and third round survey involving 14 of the original 26 experts. We report here the consolidated results of the entire procedure. There was uniform agreement on several general statements, including that PSAs should be performed in hemostasis laboratories as first line diagnostic tests even in patients with normal platelet aggregation, and should include a delta-granule secretion marker. Among the specific assays examined, lumiaggregometry, other luciferin/luciferase-based assays, high-performance liquid chromatography methods, radiolabeled-serotonin based assays, and whole-mount transmission electron microscopy were rated as appropriate for the measurement of delta-granule release, and platelet P-selectin expression by flow cytometry and released proteins by ELISA for alpha-granule release. For most of the other PSAs, the expert opinions were widely dispersed. Lack of expert consensus on many PSAs clearly indicates an unmet need for rigorous standardization, multicenter comparison of results, and validation of PSAs for clinical laboratory practice.
dc.description.funderANID-FONDECYT
dc.fuente.origenWOS
dc.identifier.doi10.1111/jth.15781
dc.identifier.eissn1538-7836
dc.identifier.issn1538-7933
dc.identifier.urihttps://doi.org/10.1111/jth.15781
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93278
dc.identifier.wosidWOS:000818838500001
dc.issue.numero9
dc.language.isoen
dc.pagina.final2135
dc.pagina.inicio2127
dc.revistaJournal of thrombosis and haemostasis
dc.rightsacceso restringido
dc.subjectexpert consensus
dc.subjectinherited platelet disorders
dc.subjectplatelet function
dc.subjectplatelet granules
dc.subjectplatelet release reaction
dc.subjectplatelet secretion
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExpert opinion on the use of platelet secretion assay for the diagnosis of inherited platelet function disorders: Communication from the ISTH SSC Subcommittee on Platelet Physiology
dc.typeartículo
dc.volumen20
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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