Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery

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dc.article.number54
dc.catalogadorpva
dc.contributor.authorCarvajal Díaz, Felipe Alonso
dc.contributor.authorVargas Torres, Valentina Isabel
dc.contributor.authorBecerra, Daniela
dc.contributor.authorGonzález Quezada, Nicolás Marcelo Orlando
dc.contributor.authorEgaña, José T.
dc.date.accessioned2025-06-10T14:58:16Z
dc.date.available2025-06-10T14:58:16Z
dc.date.issued2025
dc.date.updated2025-06-08T00:08:26Z
dc.description.abstractBackgroud Genetically engineered photosynthetic microorganisms have been proposed as a therapeutic approach for the localized delivery of oxygen and recombinant proteins to tissues in various pathological conditions. However, the effect of recombinant protein production and secretion on microalgal fitness, as well as the impact of key environmental conditions on their potential therapeutic performance, has not yet been described. Therefore, in this study, the microalga Chlamydomonas reinhardtii was genetically engineered to produce and release the reporter protein mVenus and was then challenged by exposure to different media, temperatures, and substrates. Results The genetically modified microalgae were able to produce and release the mVenus protein under standard culture conditions without affecting overall fitness, including cell size and shape, growth potential, and oxygen metabolism, compared to the wild-type strain. Under mammalian cell culture conditions, the strains continued to produce and secrete mVenus protein for up to four days at 22 °C, 30 °C, and 37 °C. Additionally, photosynthetic biomaterials containing the engineered microalgae showed continuous recombinant protein release at 30 °C and 37 °C for up to four days. Conclusion The microalga Chlamydomonas reinhardtii can be genetically engineered to produce and release recombinant proteins without detrimental effects on its fitness, showing therapeutic potential under mammalian culture conditions and within biomaterials designed to promote tissue regeneration. Overall, these findings support the use of genetically engineered photosynthetic microalgae for the localized and controlled release of oxygen and recombinant proteins for several therapeutic applications.
dc.fechaingreso.objetodigital2025-06-08
dc.format.extent17 páginas
dc.fuente.origenBiomed Central
dc.identifier.citationJournal of Biological Engineering. 2025 Jun 04;19(1):54
dc.identifier.doi10.1186/s13036-025-00525-9
dc.identifier.issn1754-1611
dc.identifier.urihttps://doi.org/10.1186/s13036-025-00525-9
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104624
dc.information.autorucFacultad de Ciencias Biológicas; Carvajal Díaz, Felipe Alonso; S/I; 171996
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Vargas Torres, Valentina Isabel; S/I; 247359
dc.information.autorucEscuela de Química; González Quezada, Nicolás Marcelo Orlando; S/I; 1066474
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Egaña, José T.; 0000-0003-2624-6992; 1018715
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherSpringer Nature
dc.revistaJournal of Biological Engineering
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGenetically engineered microalgae
dc.subjectRecombinant protein production
dc.subjectTherapeutic oxygenation
dc.subjectMicroalgal fitness
dc.subjectLocalized protein delivery
dc.subjectChlamydomonas reinhardtii
dc.subject.ddc570
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery
dc.typeartículo
dc.volumen19
sipa.codpersvinculados171996
sipa.codpersvinculados247359
sipa.codpersvinculados1066474
sipa.codpersvinculados1018715
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