UTERINE KALLIKREIN IN THE EARLY PREGNANT RAT

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dc.contributor.authorVALDES, G
dc.contributor.authorCORTHORN, J
dc.contributor.authorSCICLI, AG
dc.contributor.authorGAETE, V
dc.contributor.authorSOTO, J
dc.contributor.authorORTIZ, ME
dc.contributor.authorFORADORI, A
dc.contributor.authorSAED, GM
dc.date.accessioned2024-01-10T13:09:53Z
dc.date.available2024-01-10T13:09:53Z
dc.date.issued1993
dc.description.abstractUterine homogenates of cycling and early pregnant Sprague Dawley rats and purified rat urinary kallikrein showed similar curves of displacement of I-125-kallikrein binding to a polyclonal antibody. Uterine kallikrein concentration measured by RIA was 8.7 +/- 2 SEM ng/g wet weight during the cycle (n = 6 in diestrus and metestrus) and 20.8 +/- 2 SEM (n = 7) ng/g wet weight on Day 7 of pregnancy (P7) (p < 0.001). On P7, kallikrein concentration was increased 12.4-fold in the implantation nodes, as compared to the interimplantation segments. Uterine homogenates of rats on P7, submitted to DEAE-cellulose chromatography and Sephadex gel filtration, yielded two fractions containing kallikrein immunoreactivity and kininogenase activity, with molecular masses that ranged from 120-125 kDa and 39-43 kDa, respectively. In the RIA, both fractions displayed parallelism with purified kallikrein. Enzymatic activity was expressed after activation by trypsin. It was inhibited by aprotinin, PMSF, p-amino-benzamidine, and leupeptin, but not by soybean or ovomucoid trypsin inhibitors. Kallikrein mRNA was demonstrated by reverse transcriptase/polymerase chain reaction in uteri of nonpregnant and P7 rats. These results show that rat uterus synthesizes one or more serine proteases that are immunologically and enzymatically related to tissue kallikrein. The increase of kallikrein in the implantation node on P7-determined both by an increment of whole uterus kallikrein content and a depletion of the interimplantation segments-suggests that kallikrein may play a role in the vasoactive changes of implantation.
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1095/biolreprod49.4.802
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.pubmedidMEDLINE:8218645
dc.identifier.urihttps://doi.org/10.1095/biolreprod49.4.802
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77701
dc.identifier.wosidWOS:A1993LY46700020
dc.information.autorucActividades Universitarias;Foradori A;S/I;47695
dc.information.autorucMedicina;Valdes G;S/I;98654
dc.issue.numero4
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final808
dc.pagina.inicio802
dc.publisherOXFORD UNIV PRESS INC
dc.revistaBIOLOGY OF REPRODUCTION
dc.rightsregistro bibliográfico
dc.subjectAORTIC ENDOTHELIAL-CELLS
dc.subjectTISSUE-KALLIKREIN
dc.subjectMESSENGER-RNA
dc.subjectGLANDULAR KALLIKREIN
dc.subjectPLASMINOGEN-ACTIVATOR
dc.subjectRELAXING FACTOR
dc.subjectMAST-CELLS
dc.subjectIDENTIFICATION
dc.subjectIMPLANTATION
dc.subjectHISTAMINE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleUTERINE KALLIKREIN IN THE EARLY PREGNANT RAT
dc.typeartículo
dc.volumen49
sipa.codpersvinculados47695
sipa.codpersvinculados98654
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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