STRUCTURE OF YEAST RNA-POLYMERASES DETERMINED BY ELECTRON-MICROSCOPY

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1982
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Methods were developed for the examination of yeast RNA polymerases I, II and III by EM. The size and shape of a eucaryotic RNA polymerase was established for the 1st time. The enzymes are roughly spherical in shape and compact in appearance. Their measured molecular diameters are 12.7 .+-. 0.4 and 11.0 .+-. 1.4 (SD) nm for polymerase I, 12.7 .+-. 1.1 and 12.2 .+-. 1.0 (SD) nm for polymerase II and 13.6 .+-. 0.6 and 11.5 .+-. 1.3 (SD) nm for polymerase III.
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