PRESERVATION OF HAMSTER OOCYTES TO ASSAY THE FERTILIZING-CAPACITY OF HUMAN-SPERMATOZOA

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dc.contributor.authorQUINN, P
dc.contributor.authorBARROS, C
dc.contributor.authorWHITTINGHAM, DG
dc.date.accessioned2025-01-23T19:44:07Z
dc.date.available2025-01-23T19:44:07Z
dc.date.issued1982
dc.description.abstractBetween 70 and 80% of zona-intact hamster ova survived freezing after slow cooling (.apprx. 0.3.degree. C/min) to -80.degree. C in Medium PB1 containing 1.5 or 2.0 M-DMSO before transfer to -196.degree. C. After slow warming (.apprx. 8.degree. C/min), there was no difference in survival if the DMSO [dimethyl sulfoxide] was diluted out by a slow stepwise or a rapid single addition of medium. When slow cooling was terminated -40.degree. C by direct transfer to -196.degree. C, up to 75% of the ova survived rapid warming (.apprx. 500.degree. C/min) and rapid dilution if the medium contained 2.0 M-DMSO. The survival rates were calculated on the basis of the number of thawed ova which retained their normal morphological appearance after a 1 h incubation before removal of the zona pellucida with trypsin. All of these ova were penetrated after incubation with mouse spermatozoa, indicating that the freezing procedure per se does not adversely affect the penetration of frozen-thawed hamster ova by heterologous spermatozoa. There was no difference in the penetration rate of human spermatozoa into frozen (34%) or fresh (42%) oocytes when a Hepes[N-2-hydroxyethylpiperazine-N''-2-ethanesulfonic-acid]-buffered Tyrode solution, containing 30 mg BSA[bovine serum albumin]/ml and 2.0 M-DMSO, was used as the freezing medium. However, fewer ova frozen in Medium PB1, containing 4 mg BSA/ml and 2.0 M-DMSO, were penetrated by human spermatozoa (18%) compared with freshly collected ova (38%). Zona-free ova did not survive the freezing procedure as well as zona-intact ova. The survival of hamster oocytes stored at -196.degree. C offers a convenient means of supplying and transporting these ova for the assessment of the fertilizing capacity of human and other heterologous spermatozoa.
dc.fuente.origenWOS
dc.identifier.issn0022-4251
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99946
dc.identifier.wosidWOS:A1982PG63500026
dc.issue.numero1
dc.language.isoen
dc.pagina.final168
dc.pagina.inicio161
dc.revistaJournal of reproduction and fertility
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePRESERVATION OF HAMSTER OOCYTES TO ASSAY THE FERTILIZING-CAPACITY OF HUMAN-SPERMATOZOA
dc.typeartículo
dc.volumen66
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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