Functionalization of stable fuorescent nanodiamonds towards reliable detection of biomarkers for Alzheimer’s disease

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dc.contributor.authorCasanova Morales, Nathalie
dc.contributor.authorGonzález, Raúl B.
dc.contributor.authorChandía Cristi, América.
dc.contributor.authorAlvizú Fiedler, Ignacio Ernesto.
dc.contributor.authorWaselowski Butikofer, Víctor Hugo.
dc.contributor.authorGuzmán, Fanny.
dc.contributor.authorOyarzún Olave, Marisol.
dc.contributor.authorRebolledo, Cristian.
dc.contributor.authorRodríguez, Enrique.
dc.contributor.authorArmijo, Julien.
dc.date.accessioned2019-10-17T15:23:17Z
dc.date.available2019-10-17T15:23:17Z
dc.date.issued2018
dc.date.updated2019-10-14T18:42:56Z
dc.description.abstractAbstract Background Stable and non-toxic fluorescent markers are gaining attention in molecular diagnostics as powerful tools for enabling long and reliable biological studies. Such markers should not only have a long half-life under several assay conditions showing no photo bleaching or blinking but also, they must allow for their conjugation or functionalization as a crucial step for numerous applications such as cellular tracking, biomarker detection and drug delivery. Results We report the functionalization of stable fluorescent markers based on nanodiamonds (NDs) with a bifunctional peptide. This peptide is made of a cell penetrating peptide and a six amino acids long β-sheet breaker peptide that is able to recognize amyloid β (Aβ) aggregates, a biomarker for the Alzheimer disease. Our results indicate that functionalized NDs (fNDs) are not cytotoxic and can be internalized by the cells. The fNDs allow ultrasensitive detection (at picomolar concentrations of NDs) of in vitro amyloid fibrils and amyloid aggregates in AD mice brains. Conclusions The fluorescence of functionalized NDs is more stable than that of fluorescent markers commonly used tostain Aβ aggregates such as Thioflavin T. These results pave the way for performing ultrasensitive and reliable detection of Aβ aggregates involved in the pathogenesis of the Alzheimer disease.
dc.fuente.origenBiomed Central
dc.identifier.citationJournal of Nanobiotechnology. 2018 Aug 10;16(1):60
dc.identifier.doi10.1186/s12951-018-0385-7
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26768
dc.identifier.wosidWOS:000441271800001
dc.issue.numeroNo. 60
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final14
dc.pagina.inicio1
dc.revistaJournal of Nanobiotechnologyes_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherEnfermedad de Alzheimeres_ES
dc.subject.otherEnfermedad de Alzheimer - Diagnósticoes_ES
dc.subject.otherDiagnóstico por imagenes_ES
dc.titleFunctionalization of stable fuorescent nanodiamonds towards reliable detection of biomarkers for Alzheimer’s disease
dc.typeartículo
dc.volumenVol. 16
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