Activation of individual L1 retrotransposon instances is restricted to cell-type dependent permissive loci

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dc.contributor.authorPhilippe, Claude
dc.contributor.authorVargas-Landin, Dulce B.
dc.contributor.authorDoucet, Aurelien J.
dc.contributor.authorvan Essen, Dominic
dc.contributor.authorVera-Otarola, Jorge
dc.contributor.authorKuciak, Monika
dc.contributor.authorCorbin, Antoine
dc.contributor.authorNigumann, Pilvi
dc.contributor.authorCristofari, Gael
dc.date.accessioned2025-01-23T21:31:46Z
dc.date.available2025-01-23T21:31:46Z
dc.date.issued2016
dc.description.abstractLINE-1 (L1) retrotransposons represent approximately one sixth of the human genome, but only the human-specific L1HS-Ta subfamily acts as an endogenous mutagen in modern humans, reshaping both somatic and germline genomes. Due to their high levels of sequence identity and the existence of many polymorphic insertions absent from the reference genome, the transcriptional activation of individual genomic L1HS-Ta copies remains poorly understood. Here we comprehensively mapped fixed and polymorphic L1HS-Ta copies in 12 commonly-used somatic cell lines, and identified transcriptional and epigenetic signatures allowing the unambiguous identification of active L1HS-Ta copies in their genomic context. Strikingly, only a very restricted subset of L1HS-Ta loci - some being polymorphic among individuals - significantly contributes to the bulk of L1 expression, and these loci are differentially regulated among distinct cell lines. Thus, our data support a local model of L1 transcriptional activation in somatic cells, governed by individual-, locus-, and cell-type-specific determinants.
dc.fuente.origenWOS
dc.identifier.doi10.7554/eLife.13926
dc.identifier.issn2050-084X
dc.identifier.urihttps://doi.org/10.7554/eLife.13926
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101476
dc.identifier.wosidWOS:000380164000001
dc.language.isoen
dc.revistaElife
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleActivation of individual L1 retrotransposon instances is restricted to cell-type dependent permissive loci
dc.typeartículo
dc.volumen5
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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