Tolerance and Metabolization of High-Concentration Heavy Crude Oil High-Concentration Heavy Crude Oil by Bacillus subtilis

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dc.catalogadorgrr
dc.contributor.authorSaez Navarrete, Cesar Antonio
dc.contributor.authorCáceres-Zambrano, Jessica Zerimar
dc.date.accessioned2025-07-03T14:59:23Z
dc.date.available2025-07-03T14:59:23Z
dc.date.issued2025
dc.description.abstractIn this comprehensive study, we investigated the degradation capacity and tolerance of the bacterial strain Bacillus subtilis in culture media with high concentrations of heavy crude oil (HCO) as the sole carbon source. Using a meticulously designed experimental approach conducted at room temperature (25 °C), we systematically examined various culture media with HCO concentrations of 20%, 35%, and 50% v/v over a 10-week period. The results revealed the microorganism’s remarkable resistance to these HCO concentrations. Biotransformation capacity was confirmed by quantifying CO2 production via gas chromatography, showing substantial bioconversion with a 42% increase in CO2 production. Additionally, changes in surface tension were monitored using the Du Noüy ring method, showing a reduction in the aqueous phase tension from 72.3 to 47.43 mN/m. At the end of the bioconversion period, all treated samples exhibited visible emulsification, indicative of biosurfactant production. This phenomenon was consistent with the observed decrease in surface tension, providing further evidence of biosurfactant-mediated mechanisms. These findings highlight the immense biotechnological potential of B. subtilis to address HCO-related challenges, offering promising prospects for crude oil bioremediation and bioupgrading.
dc.fechaingreso.objetodigital2025-07-03
dc.format.extent15 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3390/microorganisms13071520
dc.identifier.issn2076-2607
dc.identifier.urihttps://doi.org/10.3390/microorganisms13071520
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104836
dc.information.autorucEscuela de Ingeniería; Saez Navarrete, Cesar Antonio; 0000-0002-3940-143X; 104647
dc.issue.numero7
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaMicroorganisms
dc.rightsacceso abierto
dc.rights.licenseCC BY 4.0 Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.en
dc.subjectHeavy crude metabolization
dc.subjectBioupgrading
dc.subjectBioconversion
dc.subjectBiotensioactives biotransformation capacity
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.titleTolerance and Metabolization of High-Concentration Heavy Crude Oil High-Concentration Heavy Crude Oil by Bacillus subtilis
dc.typeartículo
dc.volumen13
sipa.codpersvinculados104647
sipa.trazabilidadORCID;2025-06-30
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