Proteomic Profile of Urinary Extracellular Vesicles Identifies AGP1 as a Potential Biomarker of Primary Aldosteronism

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dc.contributor.authorBarros, Eric R.
dc.contributor.authorRigalli, Juan Pablo
dc.contributor.authorTapia-Castillo, Alejandra
dc.contributor.authorVecchiola, Andrea
dc.contributor.authorYoung, Morag J.
dc.contributor.authorHoenderop, Joost G. J.
dc.contributor.authorBindels, Rene J. M.
dc.contributor.authorFardella, Carlos E.
dc.contributor.authorCarvajal, Cristian A.
dc.date.accessioned2025-01-20T23:51:55Z
dc.date.available2025-01-20T23:51:55Z
dc.date.issued2021
dc.description.abstractContext: Primary aldosteronism (PA) represents 6% to 10% of all essential hypertension patients and is diagnosed using the aldosterone-to-renin ratio (ARR) and confirmatory studies. The complexity of PA diagnosis encourages the identification of novel PA biomarkers. Urinary extracellular vesicles (uEVs) are a potential source of biomarkers, considering that their cargo reflects the content of the parent cell.
dc.description.abstractObjective: We aimed to evaluate the proteome of uEVs from PA patients and identify potential biomarker candidates for PA.
dc.description.abstractMethods: Second morning spot urine was collected from healthy controls (n = 8) and PA patients (n = 7). The uEVs were isolated by ultracentrifugation and characterized. Proteomic analysis on uEVs was performed using LC-MS Orbitrap.
dc.description.abstractResults: Isolated uEVs carried extracellular vesicle markers, showed a round shape and sizes between 50 and 150 nm. The concentration of uEVs showed a direct correlation with urinary creatinine (r = 0.6357; P= 0.0128).The uEV size mean (167 +/- 6 vs 183 +/- 4nm) and mode (137 +/- 7 vs 171 +/- 11nm) was significantly smaller in PA patients than in control subjects, but similar in concentration. Proteomic analysis of uEVs from PA patients identified an upregulation of alpha-1-acid glycoprotein 1 (AGP1) in PA uEVs, which was confirmed using immunoblot. A receiver operating characteristic curve analysis showed an area under the curve of 0.92 (0.82 to 1; P. 0.0055).
dc.description.abstractConclusion: Proteomic and further immunoblot analyses of uEVs highlights AGP1 as potential biomarker for PA.
dc.fuente.origenWOS
dc.identifier.doi10.1210/endocr/bqab032
dc.identifier.eissn1945-7170
dc.identifier.issn0013-7227
dc.identifier.urihttps://doi.org/10.1210/endocr/bqab032
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94874
dc.identifier.wosidWOS:000628736500016
dc.issue.numero4
dc.language.isoen
dc.revistaEndocrinology
dc.rightsacceso restringido
dc.subjectprimary aldosteronism urine
dc.subjectextracellular vesicles
dc.subjectproteomics
dc.subjecthypertension
dc.subjectaldosterone-to-renin ratio
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleProteomic Profile of Urinary Extracellular Vesicles Identifies AGP1 as a Potential Biomarker of Primary Aldosteronism
dc.typeartículo
dc.volumen162
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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