Antifungal properties of quinoa (<i>Chenopodium quinoa</i> Willd) alkali treated saponnins against <i>Botrytis cinerea</i>

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dc.contributor.authorStuardo, Macarena
dc.contributor.authorMartin, Ricardo San
dc.date.accessioned2025-01-21T01:05:06Z
dc.date.available2025-01-21T01:05:06Z
dc.date.issued2008
dc.description.abstractQuinoa (Chenopodium quinoa Willd) is a Latin American food staple readily available in large quantities in Peru, Bolivia and Ecuador. The outer husk of the grain is removed prior to consumption to reduce its bitter taste. At present, quinoa husks are considered as a by-product with no commercial value, despite its high content of triterpenoid saponins (20-30%). Due to this, the present work was undertaken to test if quinoa saponins have antifungal properties against Botrytis cinerea and if this activity is enhanced after alkaline treatment, since recent reports indicate that alkaline treatment of quinoa saponins increase their biological activity. Six products were tested against B. cinerea: (1) non-purified quinoa extract, (2) purified quinoa extract, (3) alkali treated non-purified quinoa extract, (4) alkali treated purified quinoa extract, (5) non-purified quinoa extract treated with alkali but without thermal incubation and (6) purified quinoa extract treated with alkali but without thermal incubation. Untreated quinoa extracts showed minimum activity against mycelial growth of B. cinerea. Also, no effects were observed against conidial germination, even at 7 mg saponins/ml. However, when the saponin extracts were treated with alkali, mycelial growth and conidial germination were significantly inhibited. At doses of 5 mg saponins/ml, 100% of conidial germination inhibition was observed, even after 96 h of incubation. Fungal membrane integrity experiments based on the uptake of the fluorogenic dye SYTOX green showed that alkali treated saponins generate membrane disruption, while non-treated saponins had no effects. The higher antifungal activity of alkaline treated saponins is probably due to the formation of more hydrophobic saponin derivatives that may have a higher affinity with the sterols present in cell membranes. (c) 2007 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.indcrop.2007.11.003
dc.identifier.eissn1872-633X
dc.identifier.issn0926-6690
dc.identifier.urihttps://doi.org/10.1016/j.indcrop.2007.11.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95819
dc.identifier.wosidWOS:000255467100010
dc.issue.numero3
dc.language.isoen
dc.pagina.final302
dc.pagina.inicio296
dc.revistaIndustrial crops and products
dc.rightsacceso restringido
dc.subjectantifungal properties
dc.subjectBotrytis cinerea
dc.subjectsaponins
dc.subjectChenopodium quinoa
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAntifungal properties of quinoa (<i>Chenopodium quinoa</i> Willd) alkali treated saponnins against <i>Botrytis cinerea</i>
dc.typeartículo
dc.volumen27
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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