ELECTRON-MICROSCOPY MAPPING OF ESCHERICHIA-COLI RNA POLYMERASE-BINDING SITES ON PLASMIDS FROM THERMOPHILIC BACTERIA

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dc.contributor.authorGONZALEZ, B
dc.contributor.authorVASQUEZ, C
dc.contributor.authorBULL, P
dc.contributor.authorVICUNA, R
dc.date.accessioned2025-01-23T19:43:51Z
dc.date.available2025-01-23T19:43:51Z
dc.date.issued1984
dc.description.abstractThe binding sites of E. coli RNA polymerase to plasmid DNA from extremely thermophilic bacteria were mapped by EM. Templates used in these studies included plasmids pTF62 (from Thermus flavus AT62) and pTT8 (from T. thermophilus HB8) and hybrid molecules constructed by ligation of these plasmids to pBR322. Although the affinity of the enzyme for heterologous DNA was .apprx. 1/3 of that for pBR322, it was possible to localize preferred binding sites on pTF62 and pTT8. Six binding sites were identified in pTT8, mapping close to 7, 28, 47, 61, 65 and 81 map units (1 unit = 1% of the length of the DNA). Seven such regions located at 3, 27, 48, 60, 67, 81 and 86 map units were found in pTF62. RNA polymerase binding sites found in pBR322 coincided with promoters identified previously by EM analysis of transcriptional complexes prepared in vitro. Evidently, E. coli RNA polymerase binds preferentially to specific sequences in plasmids from thermophilic bacteria, suggesting possible promoter locations in these plasmids.
dc.fuente.origenWOS
dc.identifier.issn0198-0238
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99842
dc.identifier.wosidWOS:A1984TA61900005
dc.issue.numero3
dc.language.isoen
dc.pagina.final257
dc.pagina.inicio251
dc.revistaDna-a journal of molecular & cellular biology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleELECTRON-MICROSCOPY MAPPING OF ESCHERICHIA-COLI RNA POLYMERASE-BINDING SITES ON PLASMIDS FROM THERMOPHILIC BACTERIA
dc.typeartículo
dc.volumen3
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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