Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model

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dc.contributor.authorSantana-Garrido, Alvaro
dc.contributor.authorReyes-Goya, Claudia
dc.contributor.authorEspinosa-Martin, Pablo
dc.contributor.authorSobrevia, Luis
dc.contributor.authorBeltran, Luis M.
dc.contributor.authorVazquez, Carmen M.
dc.contributor.authorMate, Alfonso
dc.date.accessioned2025-01-20T21:02:55Z
dc.date.available2025-01-20T21:02:55Z
dc.date.issued2022
dc.description.abstractPreeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclampsia induced by excess sFlt1 (soluble fms-like tyrosine kinase 1). Placenta and kidney samples were obtained from normal pregnant and PE rats to measure the sFlt1/PlGF (placental growth factor) ratio in addition to oxidative stress-related parameters, including the activities and expressions of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), components of nitric oxide (NO) metabolism, and antioxidant enzymes. Peroxisome proliferator-activated receptors (PPAR alpha, PPAR gamma) and cytokines IL1 beta, IL3, IL6, IL10, and IL18 were also measured to evaluate the inflammation status in our experimental setting. Excessive O-2(?-) production was found in rats that were treated with sFlt1; interestingly, this alteration appears to be mediated mainly by NOX2 in the placenta and by NOX4 in the kidney. Altered NO metabolism and antioxidant defense systems, together with mitochondrial dysfunction, were observed in this model of PE. Preeclamptic animals also exhibited overexpression of proinflammatory biomarkers as well as increased collagen deposition. Our results highlight the role of NADPH oxidase in mediating oxidative stress and possibly inflammatory processes in the placenta and kidney of an sFlt1-based model of early-onset preeclampsia.
dc.fuente.origenWOS
dc.identifier.doi10.3390/antiox11081608
dc.identifier.eissn2076-3921
dc.identifier.urihttps://doi.org/10.3390/antiox11081608
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93100
dc.identifier.wosidWOS:000846380700001
dc.issue.numero8
dc.language.isoen
dc.revistaAntioxidants
dc.rightsacceso restringido
dc.subjectinflammation
dc.subjectkidney
dc.subjectNADPH oxidase
dc.subjectnitric oxide
dc.subjectoxidative stress
dc.subjectplacenta
dc.subjectpreeclampsia
dc.subjectsFlt1
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleOxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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