Serotonin secretion by blood platelets: accuracy of high-performance liquid chromatography-electrochemical technique compared with the isotopic test and use in a clinical laboratory

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dc.contributor.authorAranda, Eduardo
dc.contributor.authorIha, Seiki
dc.contributor.authorSolari, Sandra
dc.contributor.authorRodriguez, David
dc.contributor.authorRomero, Viviana
dc.contributor.authorVillarroel, Luis
dc.contributor.authorPereira, Jaime
dc.contributor.authorPanes, Olga
dc.contributor.authorMezzano, Diego
dc.date.accessioned2025-01-20T17:37:37Z
dc.date.available2025-01-20T17:37:37Z
dc.date.issued2023
dc.description.abstractBackground: Mild secretion defects are the most frequent and challenging blood platelet disorders to diagnose. Most d-granule secretion tests lack validation, are not quantitative, or have unreliable response to weak platelet agonists.
dc.description.abstractObjectives: To compare platelet serotonin secretion by HPLC-electrochemical detection technique (HPLC-ECD) with the reference isotopic test (3H-5-HT), evaluating its performance in clinical laboratories.
dc.description.abstractMethods: The assay validation followed STARD-2015 recommendations. HPLC-ECD measured the nonsecreted serotonin remaining in platelet pellets after aggregation, comparing it with the reference H-3-5-HT assay. We studied subjects with inherited and aspirin-induced blood platelet disorders and assessed the HPLC-ECD operation for routine clinical diagnosis.
dc.description.abstractResults: Calibration curves were linear (R-2 = 0.997), with SD for residuals of 3.91% and analytical sensitivity of 5ng/mL. Intra- and interassay imprecision bias ranged between -8.5% and 2.1% and -9% and 3.1%, respectively. Serotonin recovery and stability were >95%, and the variability range of measurements was -5.5% to 4.6%. Statistical differences detected between tests were biologically irrelevant, with bias of 1.48% (SD, 8.43) and CI agreement of -18% to 15%. Both assays distinctly detected platelet secretion induced by 10 mu M epinephrine and 4 mu mM adenosine diphosphate. However, HPLC-ECD is quantitative and more sensitive to low serotonin content in blood platelets. Reference cutoffs for each agonist were determined in 87 subjects. Initially, the HPLC-ECD requires relatively expensive equipment and trained operators but has remarkably cheap running costs and a turn-around time of 24-36 hours. We have used this diagnostic tool routinely for >8 years.
dc.description.abstractConclusion: HPLC-ECD assay for platelet serotonin secretion is highly accurate, has advantages over the reference H-3-5-HT test, and is suitable as a clinical laboratory technique.
dc.description.funderANID-FONDECYT, Chile
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.rpth.2023.102156
dc.identifier.eissn2475-0379
dc.identifier.urihttps://doi.org/10.1016/j.rpth.2023.102156
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91695
dc.identifier.wosidWOS:001061602100001
dc.issue.numero5
dc.language.isoen
dc.revistaResearch and practice in thrombosis and haemostasis
dc.rightsacceso restringido
dc.subjectblood platelets
dc.subjectclinical laboratory techniques
dc.subjectelectrochemical technique
dc.subjectplatelet aggregation
dc.subjectplatelet secretion
dc.subjectserotonin
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSerotonin secretion by blood platelets: accuracy of high-performance liquid chromatography-electrochemical technique compared with the isotopic test and use in a clinical laboratory
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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