The enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species: consequences for NADPH production

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dc.catalogadorgjm
dc.contributor.authorFuentes Lemus, Eduardo Felipe
dc.contributor.authorReyes Valenzuela, Juan Sebastián
dc.contributor.authorFigueroa Alegría, Juan David
dc.contributor.authorDavies, Michael J.
dc.contributor.authorLópez Alarcón, Camilo Ignacio
dc.date.accessioned2024-12-30T16:25:36Z
dc.date.available2024-12-30T16:25:36Z
dc.date.issued2023
dc.description.abstractThe pentose phosphate pathway (PPP) is a key metabolic pathway. The oxidative phase of this process involves three reactions catalyzed by glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL) and 6-phosphogluconate dehydrogenase (6PGDH) enzymes. The first and third steps (catalyzed by G6PDH and 6PGDH, respectively) are responsible for generating reduced nicotinamide adenine dinucleotide phosphate (NAPDH), a key cofactor for maintaining the reducing power of cells and detoxification of both endogenous and exogenous oxidants and electrophiles. Despite the importance of these enzymes, little attention has been paid to the fact that these proteins are targets of oxidants. In response to oxidative stimuli metabolic pathways are modulated, with the PPP often up-regulated in order to enhance or maintain the reductive capacity of cells. Under such circumstances, oxidation and inactivation of the PPP enzymes could be detrimental. Damage to the PPP enzymes may result in a downward spiral, as depending on the extent and sites of modification, these alterations may result in a loss of enzymatic activity and therefore increased oxidative damage due to NADPH depletion. In recent years, it has become evident that the three enzymes of the oxidative phase of the PPP have different susceptibilities to inactivation on exposure to different oxidants. In this review, we discuss existing knowledge on the role that these enzymes play in the metabolism of cells, and their susceptibility to oxidation and inactivation with special emphasis on NADPH production. Perspectives on achieving a better understanding of the molecular basis of the oxidation these enzymes within cellular environments are given.
dc.fuente.origenScopus
dc.identifier.doi10.1042/bst20231027
dc.identifier.issn1470-8752
dc.identifier.scopusidSCOPUS_ID:85178114166
dc.identifier.urihttps://doi.org/10.1042/BST20231027
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89375
dc.information.autorucEscuela de Química; Fuentes Lemus, Eduardo Felipe; 0000-0002-1465-8466; 186720
dc.information.autorucEscuela de Química; Reyes Valenzuela, Juan Sebastián; S/I; 1268701
dc.information.autorucEscuela de Química; Figueroa Alegría, Juan David; S/I; 1071087
dc.information.autorucEscuela de Química; López Alarcón, Camilo Ignacio; S/I; 1004308
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final2187
dc.pagina.inicio2173
dc.revistaBiochemical Society Transactions
dc.rightsacceso restringido
dc.subject6-phosphogluconate dehydrogenase
dc.subject6-phosphogluconolactonase
dc.subjectglucose-6-phosphate dehydrogenase
dc.subjectNADPH
dc.subjectpentose phosphate pathway
dc.subjectprotein oxidation
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species: consequences for NADPH production
dc.typeartículo
dc.volumen51
sipa.codpersvinculados186720
sipa.codpersvinculados1268701
sipa.codpersvinculados1071087
sipa.codpersvinculados1004308
sipa.trazabilidadSCOPUS;2023-12-10
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