A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity

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dc.catalogadoraba
dc.contributor.authorLarrondo Castro, Luis Fernando
dc.contributor.authorSalas, L.
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorVicuña, Rafael
dc.contributor.authorCullen, D.
dc.date.accessioned2025-02-05T19:01:01Z
dc.date.available2025-02-05T19:01:01Z
dc.date.issued2003
dc.description.abstractLignin degradation by the white rot basidiomycete Phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese peroxidase, and a peroxide-generating enzyme, glyoxal oxidase. Recent studies have suggested that laccases also may be produced by this fungus, but these conclusions have been controversial. We identified four sequences related to laccases and ferroxidases (Fet3) in a search of the publicly available P. chrysosporium database. One gene, designated mco1, has a typical eukaryotic secretion signal and is transcribed in defined media and in colonized wood. Structural analysis and multiple alignments identified residues common to laccase and Fet3 sequences. A recombinant MCO1 (rMCO1) protein expressed in Aspergillus nidulans had a molecular mass of 78 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the copper I-type center was confirmed by the UV-visible spectrum. rMCO1 oxidized various compounds, including 2,2′-azino(bis-3-ethylbenzthiazoline-6-sulfonate) (ABTS) and aromatic amines, although phenolic compounds were poor substrates. The best substrate was Fe2+, with a Km close to 2 μM. Collectively, these results suggest that the P. chrysosporium genome does not encode a typical laccase but rather encodes a unique extracellular multicopper oxidase with strong ferroxidase activity.
dc.fuente.origenSIPA
dc.identifier.doi10.1128/AEM.69.10.6257-6263.2003
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.pubmedid14532088
dc.identifier.pubmedidPMC201228
dc.identifier.scopusid2-s2.0-0142040829
dc.identifier.urihttps://doi.org/10.1128/AEM.69.10.6257-6263.2003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102154
dc.identifier.wosidWOS:000185881300066
dc.information.autorucFacultad de Ciencias Biológicas; Larrondo Castro, Luis Fernando; 0000-0002-8832-7109; 3629
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.information.autorucFacultad de Ciencias Biológicas; Vicuña, Rafael; S/I; 99063
dc.issue.numero10
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final6263
dc.pagina.inicio6257
dc.revistaApplied and environmental microbiology
dc.rightsacceso restringido
dc.subjectIntergenic Region
dc.subjectUniGene Cluster
dc.subjectUnambiguous Assignment
dc.subjectAnnotate Transcript
dc.subjectSaccharomyces Genome Database
dc.subject.ddc570
dc.subject.deweyBiología
dc.subject.ods06 Clean water and sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleA novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity
dc.typeartículo
dc.volumen69
sipa.codpersvinculados3629
sipa.codpersvinculados82342
sipa.codpersvinculados99063
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