Subcellular localization of catalase in sea urchin (<i>Tetrapigus niger</i>) gametes

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dc.contributor.authorFigueroa, C
dc.contributor.authorKawada, ME
dc.contributor.authorMunizaga, A
dc.contributor.authorGonzález, S
dc.contributor.authorBarros, C
dc.contributor.authorKoenig, C
dc.contributor.authorSantos, MJ
dc.date.accessioned2025-01-21T01:33:01Z
dc.date.available2025-01-21T01:33:01Z
dc.date.issued1997
dc.description.abstractPeroxisomes are essential subcellular organelles that appear to be derived from pre existing organelles. To test the presence of peroxisomes in sea urchin (Tetrapigus niger) sperm and eggs, we performed biochemical and morphological experiments to evaluate the subcellular distribution of catalase as the typical peroxisomal marker. In sea urchin sperm, we found that catalase is localized in the cell cytosol. In contrast, sea urchin eggs contain sedimentable catalase, presumably contained in peroxisome-like structures detected by immunomicroscopy and by cytochemistry. Our results show, for the first time, evidence for the presence of peroxisome-like structures in sea urchin eggs and provide evidence for the peroxisome biogenesis hypothesis by division of preexisting organelles. (C) 1997 Elsevier Science Inc.
dc.fuente.origenWOS
dc.identifier.issn0305-0491
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97358
dc.identifier.wosidWOS:000071220000004
dc.issue.numero4
dc.language.isoen
dc.pagina.final763
dc.pagina.inicio757
dc.revistaComparative biochemistry and physiology b-biochemistry & molecular biology
dc.rightsacceso restringido
dc.subjectperoxisome
dc.subjectcatalase
dc.subjectoocytes
dc.subjectsperm
dc.subjectsea urchin
dc.subjectbiogenesis
dc.subjectsubcellular fractionation
dc.subjectimmunodetection
dc.subjectconfocal microscopy
dc.subjectreflectance microscopy
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSubcellular localization of catalase in sea urchin (<i>Tetrapigus niger</i>) gametes
dc.typeartículo
dc.volumen118
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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