The Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana

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dc.article.numbere70044
dc.catalogadorgrr
dc.contributor.authorFuenzalida Valdivia, Isabel Cristina
dc.contributor.authorHerrera Vásquez, Ariel Esteban
dc.contributor.authorGangas, Maria Victoria
dc.contributor.authorSaez-Vasquez, Julio
dc.contributor.authorAlvarez, José Miguel
dc.contributor.authorMeneses Araya, Claudio Antonio
dc.contributor.authorBlanco Herrera, María Francisca
dc.date.accessioned2025-11-18T16:54:39Z
dc.date.available2025-11-18T16:54:39Z
dc.date.issued2024
dc.description.abstractIn Arabidopsis thaliana, the transcription factors WRKY7, WRKY11 and WRKY17 act as negative defence regulators against Pseudomonas syringae pv. tomato (Pst) DC3000. However, their coordinated regulation of gene expression has yet to be fully explored. In this study, we conducted a transcriptomic analysis on the triple mutant wrky7/11/17 in response to Pst DC3000 at 0, 3 and 24h post-inoculation (hpi). Our results suggest that at early infection stages (0 and 3 hpi), WRKY7, WRKY11 and WRKY17 significantly repress a group of genes involved in signal perception and transduction, including receptor-like kinases. Furthermore, at later stages of interaction (24 hpi), these transcription factors induce genes related to the biosynthesis and signalling of the jasmonic acid (JA) pathway. Further infection experiments with Pst DC3000 in plants treated with methyl jasmonate (a JA analogue) and infections with Botrytis cinerea, a pathogen against which JA-mediated responses are crucial for effective defence, support this proposal. Moreover, we analysed the role of WRKY7, WRKY11 and WRKY17 in alternative splicing regulation. A comparison between differentially expressed (DEG) and spliced (DAS) genes revealed that over 80% of DAS events do not occur in conjunction with overall changes in gene expression. Alternative splicing events were found in genes with functions in splicing and the JA pathway, such as ALY4, PRP40A, JAZ3 and JAZ10. These results suggest that WRKY7, WRKY11 and WRKY17 can also participate in this layer of gene expression regulation to modulate immunity negatively
dc.description.funderMillennium Institute Center for Genome Regulation
dc.description.funderFONDECYT
dc.description.funderAgencia Nacional de Investigación y Desarrollo
dc.fechaingreso.objetodigital2025-11-18
dc.format.extent20 páginas
dc.fuente.origenSCOPUS
dc.identifier.doi10.1111/mpp.70044
dc.identifier.eissn1364-3703
dc.identifier.eissn1364-3703
dc.identifier.issn1464-6722
dc.identifier.pubmedid39717006
dc.identifier.scopusidSCOPUS_ID:85212846050
dc.identifier.urihttps://doi.org/10.1111/mpp.70044
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/107028
dc.identifier.wosidWOS:001382153600001
dc.information.autorucFacultad de Ciencias Biológicas; Herrera Vásquez, Ariel Esteban; 0000-0003-4313-8475; 142395
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Meneses Araya, Claudio Antonio; S/I; 1246418
dc.information.autorucFacultad de Ciencias Biológicas; Blanco Herrera, María Francisca; 0000-0002-7497-9907; 12937
dc.information.autorucFacultad de Ciencias Biológicas; Fuenzalida Valdivia, Isabel Cristina; S/I; 1365737
dc.issue.numero12
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaMolecular Plant Pathology
dc.rightsacceso abierto
dc.rights.licenseCC BY-NC-ND 4.0 Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAlternative splicing
dc.subjectArabidopsis thaliana
dc.subjectImmune regulation
dc.subjectJasmonic acid
dc.subjectPseudomonas syringae
dc.subjectTranscriptomics
dc.subjectWRKY transcription factors
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana
dc.typeartículo
dc.volumen25
sipa.codpersvinculados142395
sipa.codpersvinculados1246418
sipa.codpersvinculados12937
sipa.codpersvinculados1365737
sipa.trazabilidadSCOPUS;2025-01-05
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