In vitro genotoxic evaluation of conventional bleached and biobleached softwood pulp mill effluents

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dc.contributor.authorPérez-Alzola, LP
dc.contributor.authorSantos, MJ
dc.date.accessioned2025-01-21T01:32:59Z
dc.date.available2025-01-21T01:32:59Z
dc.date.issued1997
dc.description.abstractThe effluents of pulp and paper mills contain about 300 different chemical compounds; many of them are mutagens and clastogens. Genotoxic studies have shown that chlorination stage liquors are significantly more genotoxic, in the Ames Salmonella assay, than the other process of lignin extraction, and that lyophilized effluents are genotoxic in cultured mammalian cells. Since these effluents from conventional bleaching stages are genotoxic, Chilean industries are interested in changing this process to a less toxic one, such as biobleaching using enzymes. In this study, we tested the in vitro genotoxicity of two types of effluents: an effluent obtained from a conventional radiata pine kraft-bleaching process (effluent D) and one derived from a biobleaching process with hemicellulase (effluent B). Both effluents were tested without any concentration or purification steps in the Ames Salmonella assay (TA100) and in the micronuclei (MN) and sister chromatid exchange (SCE) tests in CHO cells. The results showed that neither effluent induced base pair substitution mutations in the Ames Salmonella assay, and neither increased the micronucleus frequency in CHO cells. But, both increased the SCE frequencies in CHO cells, showing that this assay is more sensitive than the other ones, and that the two effluents contained chemical compounds in amounts enough to induce in vitro genotoxicity measured by the SCE induction. (C) 1997 Elsevier Science B.V.
dc.fuente.origenWOS
dc.identifier.eissn1879-3592
dc.identifier.issn1383-5718
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97352
dc.identifier.wosidWOS:000072513300003
dc.issue.numero2-3
dc.language.isoen
dc.pagina.final112
dc.pagina.inicio107
dc.revistaMutation research-genetic toxicology and environmental mutagenesis
dc.rightsacceso restringido
dc.subjectpaper mill effluent
dc.subjectAmes Salmonella assay
dc.subjectCHO
dc.subjectmicronuclei
dc.subjectSCE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIn vitro genotoxic evaluation of conventional bleached and biobleached softwood pulp mill effluents
dc.typeartículo
dc.volumen395
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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