A single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle

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dc.contributor.authorDiaz-Castro, Francisco
dc.contributor.authorTunon-Suarez, Mauro
dc.contributor.authorRivera, Patricia
dc.contributor.authorBotella, Javier
dc.contributor.authorCancino, Jorge
dc.contributor.authorFigueroa, Ana Maria
dc.contributor.authorGutierrez, Juan
dc.contributor.authorCantin, Claudette
dc.contributor.authorDeldicque, Louise
dc.contributor.authorZbinden-Foncea, Hermann
dc.contributor.authorNielsen, Joachim
dc.contributor.authorHenriquez-Olguin, Carlos
dc.contributor.authorMorselli, Eugenia
dc.contributor.authorCastro-Sepulveda, Mauricio
dc.date.accessioned2025-01-20T16:13:00Z
dc.date.available2025-01-20T16:13:00Z
dc.date.issued2024
dc.description.abstractAimThe present study aimed to investigate the effects of a single bout of resistance exercise on mitophagy in human skeletal muscle (SkM).MethodsEight healthy men were recruited to complete an acute bout of one-leg resistance exercise. SkM biopsies were obtained one hour after exercise in the resting leg (Rest-leg) and the contracting leg (Ex-leg). Mitophagy was assessed using protein-related abundance, transmission electron microscopy (TEM), and fluorescence microscopy.ResultsOur results show that acute resistance exercise increased pro-fission protein phosphorylation (DRP1Ser616) and decreased mitophagy markers such as PARKIN and BNIP3L/NIX protein abundance in the Ex-leg. Additionally, mitochondrial complex IV decreased in the Ex-leg when compared to the Rest-leg. In the Ex-leg, TEM and immunofluorescence images showed mitochondrial cristae abnormalities, a mitochondrial fission phenotype, and increased mitophagosome-like structures in both subsarcolemmal and intermyofibrillar mitochondria. We also observed increased mitophagosome-like structures on the subsarcolemmal cleft and mitochondria in the extracellular space of SkM in the Ex-leg. We stimulated human primary myotubes with CCCP, which mimics mitophagy induction in the Ex-leg, and found that BNIP3L/NIX protein abundance decreased independently of lysosomal degradation. Finally, in another human cohort, we found a negative association between BNIP3L/NIX protein abundance with both mitophagosome-like structures and mitochondrial cristae density in the SkM.ConclusionThe findings suggest that a single bout of resistance exercise can initiate mitophagy, potentially involving mitochondrial ejection, in human skeletal muscle. BNIP3L/NIX is proposed as a sensitive marker for assessing mitophagy flux in SkM.
dc.fuente.origenWOS
dc.identifier.doi10.1111/apha.14203
dc.identifier.eissn1748-1716
dc.identifier.issn1748-1708
dc.identifier.urihttps://doi.org/10.1111/apha.14203
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90367
dc.identifier.wosidWOS:001270555600001
dc.issue.numero9
dc.language.isoen
dc.revistaActa physiologica
dc.rightsacceso restringido
dc.subjectBNIP3L/NIX
dc.subjectmitochondria cristae
dc.subjectmitochondria dynamics
dc.subjectmitophagy
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle
dc.typeartículo
dc.volumen240
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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