Cloning and molecular analysis of a cDNA and the <i>Cs-mnp1</i> gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete <i>Ceriporiopsis subvermispora</i>

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dc.contributor.authorLobos, S
dc.contributor.authorLarrondo, L
dc.contributor.authorSalas, L
dc.contributor.authorKarahanian, E
dc.contributor.authorVicuña, R
dc.date.accessioned2025-01-21T01:32:53Z
dc.date.available2025-01-21T01:32:53Z
dc.date.issued1998
dc.description.abstractA cDNA (MnP13-1) and the Cs-mnp1 gene encoding for an isoenzyme of manganese peroxidase (MnP) from C. subvermispora were isolated separately and sequenced. The cDNA, identified in a library constructed in the vector Lambda ZIPLOX, contains 1285 nucleotides, excluding the poly(A) tail, and has a 63% G + C content. The deduced protein sequence shows a high degree of identity with MnPs from other fungi. The mature protein contains 364 amino acids, which are preceded by a 24-amino-acid leader sequence. Consistent with the peroxidase mechanism of MnP, the proximal histidine, the distal histidine and the distal arginine are conserved, although the aromatic binding site (L/V/I-P-X-P) is less hydrophilic than those of other peroxidases. A gene coding for the same protein (Cs-mnp1) was isolated from a genomic library constructed in Lambda GEM-11 vector using the cDNA MnP13-1 as a probe. A subcloned SacI fragment of 2.5 kb contained the complete sequence of the Cs-mnp1 gene, including 162 bp and 770 bp of the upstream and downstream regions, respectively. The Cs-mnp1 gene possesses seven short intervening sequences. The intron splice junction sequences as well as the putative internal lariat formation sites adhere to the GT-AG and CTRAY rules, respectively. To examine the structure of the regulatory region of the Cs-mnp1 gene further, a fragment of 1.9 kb was amplified using inverse PCR. A putative TATAA element was identified 5' of the translational start codon. Also, an inverted CCAAT element, SP-1 and AP-2 sites and several putative heat-shock and metal response elements were identified. (C) 1998 Elsevier Science B.V.
dc.fuente.origenWOS
dc.identifier.issn0378-1119
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97337
dc.identifier.wosidWOS:000072104800005
dc.issue.numero2
dc.language.isoen
dc.pagina.final193
dc.pagina.inicio185
dc.revistaGene
dc.rightsacceso restringido
dc.subjectlignocellulose biodegradation
dc.subjectwood-rotting fungi
dc.subjectcDNA and genomic clones
dc.subjectintrons
dc.subjectheat-schock element
dc.subjectmetal-response element
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleCloning and molecular analysis of a cDNA and the <i>Cs-mnp1</i> gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete <i>Ceriporiopsis subvermispora</i>
dc.typeartículo
dc.volumen206
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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