PUTATIVE GLUTAMATE RECEPTORS IN MEMBRANES OBTAINED FROM HEADS OF DROSOPHILA-MELANOGASTER

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dc.contributor.authorFIEDLER, JL
dc.contributor.authorINESTROSA, NC
dc.contributor.authorBUSTOS, G
dc.date.accessioned2025-01-23T19:26:44Z
dc.date.available2025-01-23T19:26:44Z
dc.date.issued1986
dc.description.abstractThe specific binding of L-[3H]-glutamic acid (GLU) was examined in membrane preparations derived from heads of Drosophila melanogaster. L-[3H]-GLU bound to the membrane preparation with biphasic kinetics in a reversible and saturable way. The specific binding was inhibited by Cl- and Ca2+ ions. Saturation analysis of the data fit a model of two independent binding sites with dissociation constants (KD) of 29 nM and 249 nM and corresponding binding site densities (Bmax) of 5.7 and 24.6 pmol/mg protein. A series of excitatory amino acid receptor agonists and antagonists were tested as inhibitors of L-[3H]-GLU specific binding. Some compounds almost totally abolished the specific binding, whereas others reduced the binding to 45% over the concentration range used. Among the first type of inhibitors, L-GLU was the most potent, D-aspartate (D-ASP) and D-GLU were 10 times less effective than L-GLU, whereas quisqualic acid had the lowest potency. Among the second type of inhibitors, glutamate diethyl ester, D-.alpha.-amino adipate, and N-methyl-D-aspartate showed the highest potency, and DL-2-amino-4-phosphonobutyrate was the less effective. Kainic acid and .gamma.-amino butyric acid were not able to modify at any concentration used the specific binding of L-[3H]-GLU. These data suggest the presence of putative GLU receptors in the brain of Drosophila.
dc.fuente.origenWOS
dc.identifier.eissn1097-4547
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99648
dc.identifier.wosidWOS:A1986E831400005
dc.issue.numero3
dc.language.isoen
dc.pagina.final515
dc.pagina.inicio505
dc.revistaJournal of neuroscience research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePUTATIVE GLUTAMATE RECEPTORS IN MEMBRANES OBTAINED FROM HEADS OF DROSOPHILA-MELANOGASTER
dc.typeartículo
dc.volumen16
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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