Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration

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dc.contributor.authorCalero-Cuenca, Francisco J.
dc.contributor.authorOsorio, Daniel S.
dc.contributor.authorCarvalho-Marques, Sofia
dc.contributor.authorSridhara, Sreerama Chaitanya
dc.contributor.authorOliveira, Luis M.
dc.contributor.authorJiao, Yue
dc.contributor.authorDiaz, Jheimmy
dc.contributor.authorJanota, Catia S.
dc.contributor.authorCadot, Bruno
dc.contributor.authorGomes, Edgar R.
dc.date.accessioned2025-01-20T23:50:59Z
dc.date.available2025-01-20T23:50:59Z
dc.date.issued2021
dc.description.abstractCells actively position their nuclei within the cytoplasm for multiple cellular and physiological functions.(1-3) Consequently, nuclear mispositioning is usually associated with cell dysfunction and disease, from muscular disorders to cancermetastasis.(4-7) Different cell types position their nuclei away from the leading edge during cell migration.(8-11) In migrating fibroblasts, nuclear positioning is driven by an actin retrograde flow originated at the leading edge that drives dorsal actin cables away from the leading edge. The dorsal actin cables connect to the nuclear envelope by the linker of nucleoskeleton and cytoskeleton (LINC) complex on transmembrane actin-associated nuclear (TAN) lines.(12-14) Dorsal actin cables are required for the formation of TAN lines. How dorsal actin cables are organized to promote TAN lines formation is unknown. Here, we report a role for Ctdnep1/Dullard, a nuclear envelope phosphatase,(15-22) and the actin regulator Eps8L2(23-25) on nuclear positioning and cell migration. We demonstrate that Ctdnep1 and Eps8L2 directly interact, and this interaction is important for nuclear positioning and cell migration. We also show that Ctdnep1 and Eps8L2 are involved in the formation and thickness of dorsal actin cables required for TAN lines engagement during nuclear movement. We propose that Ctdnep1-Eps8L2 interaction regulates dorsal actin cables for nuclear movement during cell migration.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.cub.2021.01.007
dc.identifier.eissn1879-0445
dc.identifier.issn0960-9822
dc.identifier.urihttps://doi.org/10.1016/j.cub.2021.01.007
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94761
dc.identifier.wosidWOS:000640072800006
dc.issue.numero7
dc.language.isoen
dc.revistaCurrent biology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCtdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
dc.typeartículo
dc.volumen31
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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