Syndecan-4 and β1 integrin are regulated by electrical activity in skeletal muscle: Implications for cell adhesion

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dc.contributor.authorUgarte, Gonzalo
dc.contributor.authorSantander, Cristian
dc.contributor.authorBrandan, Enrique
dc.date.accessioned2025-01-21T00:05:40Z
dc.date.available2025-01-21T00:05:40Z
dc.date.issued2010
dc.description.abstractSyndecan-4 and integrins are involved in the cell migration and adhesion processes in several cell types. Syndecan-4, a transmembrane heparan sulfate proteoglycan, is associated to focal adhesions in adherent cells and has been described as a marker of satellite cells in skeletal muscle. In this tissue, beta 1 integrin forms heterodimers with alpha 5 and alpha 6 during myoblast differentiation and with alpha 7 in adult muscle. Here, we show that the levels of these two cell surface membrane molecules are regulated by spontaneous electrical activity during the differentiation of rat primary myoblasts. Syndecan-4 and beta 1 integrin protein levels decrease after the inhibition of electrical activity using tetrodotoxin (TTX). Syndecan-4 also decreases substantially in denervated rat tibialis anterior muscle. Indirect immunofluorescence analysis shows that syndecan-4 and beta 1 integrin co-localize with vinculin, a molecular marker of costameres in skeletal muscle myofibers. Co-localization is lost in inactive myotubes adopting a diffuse pattern, suggesting that the costameric organization is disrupted in TTX-treated myotubes. Moreover, the inhibition of spontaneous electrical activity decreases myotube cell adhesion. In summary, this work shows that syndecan-4 and beta 1 integrin protein levels and their localization in costameric structures are regulated by electrical activity and suggests that this regulatory mechanism influences the adhesion properties of skeletal myotubes during differentiation. (C) 2010 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.matbio.2010.03.003
dc.identifier.eissn1569-1802
dc.identifier.issn0945-053X
dc.identifier.urihttps://doi.org/10.1016/j.matbio.2010.03.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95557
dc.identifier.wosidWOS:000280726400005
dc.issue.numero5
dc.language.isoen
dc.pagina.final392
dc.pagina.inicio383
dc.revistaMatrix biology
dc.rightsacceso restringido
dc.subjectSyndecans
dc.subjectHeparan sulfate proteoglycans
dc.subjectSkeletal muscle
dc.subjectCostameres
dc.subjectCell adhesion
dc.subjectTTX
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSyndecan-4 and β1 integrin are regulated by electrical activity in skeletal muscle: Implications for cell adhesion
dc.typeartículo
dc.volumen29
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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