Significantly reduced expression of the proteoglycan decorin in Alzheimer's disease fibroblasts

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dc.catalogadoraba
dc.contributor.authorBrandan, Enrique
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorGarcía, M.
dc.contributor.authorContreras, M.
dc.date.accessioned2025-02-06T19:18:39Z
dc.date.available2025-02-06T19:18:39Z
dc.date.issued1996
dc.description.abstractAims—To investigate whether proteoglycan synthesis is altered in skin fibroblasts in patients with Alzheimer's disease compared with normal subjects. Methods—Cell lines obtained from donors with Alzheimer's disease and healthy controls were incubated with radioactive sulphate. The proteoglycans synthesised were determined and analysed by chromatographic, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and glycosaminoglycans-lyase treatment. The amount of decorin synthesised by each cell line was quantified using western blot analysis. Transcripts for human decorin were determined using northern blot analysis. Results—No significant changes in total sulphate incorporation and glycos-aminoglycan (GAG) composition were detected in the incubation media of these cells. However, chromatographic and SDS-PAGE analysis of the proteoglycans secreted by the cell lines showed that a dermatan sulphate proteoglycan of 150-125 kilodaltons was substantially reduced in Alzheimer's disease fibroblasts. The molecular characteristics of this proteoglycan correspond to decorin. Western blot analysis indicated that decorin was reduced in Alzheimer's disease incubation medium compared with normal medium. Northern blotting indicated that in Alzheimer's disease fibroblasts decorin transcripts were significantly reduced compared with normal fibroblasts. Glypican concentrations, a cell surface heparan sulphate proteoglycan, remained the same. Conclusions—These results strongly suggest that the expression and synthesis of decorin is affected in Alzheimer's disease skin fibroblasts.
dc.format.extent6 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1136/mp.49.6.m351
dc.identifier.issn1355-2910
dc.identifier.pubmedid16696102
dc.identifier.pubmedidPMC408086
dc.identifier.scopusid2-s2.0-0030440316
dc.identifier.urihttps://doi.org/10.1136/mp.49.6.m351
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102178
dc.identifier.wosidWOS:A1996WA28400006
dc.information.autorucFacultad de Ciencias Biológicas; Brandan, Enrique; 0000-0002-6820-5059; 52075
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.finalM356
dc.pagina.inicioM351
dc.revistaClinical molecular pathology
dc.rightsacceso restringido
dc.subjectProteoglycans
dc.subjectDecorin
dc.subjectCell adhesion
dc.subjectAlzheimer's disease
dc.subjectFibroblasts
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSignificantly reduced expression of the proteoglycan decorin in Alzheimer's disease fibroblasts
dc.typeartículo
dc.volumen49
sipa.codpersvinculados52075
sipa.codpersvinculados82342
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