Browsing by Author "Leighton, Federico"

Now showing 1 - 14 of 14
  • Thumbnail Image
    Item
    A High-Fat Diet induces and red wine counteracts endothelial dysfuntion in human volunteers
    (2000) Cuevas, Ada; Germain Aravena, Alfredo M.; Leighton, Federico
  • Thumbnail Image
    Item
    Cholesterol and vitamin E determination in broiler chickens fed canola oil
    (2015) Gallardo Paffetti, María Asunción.; Pérez Pons, Druso Diego; Leighton, Federico
  • Thumbnail Image
    Item
  • Thumbnail Image
    Item
    Cytoprotection by Neutral Fraction of Tannat Red Wine Against Oxidative Stress-Induced Cell Death
    (2004) Echeverry, Carolina; Vásquez, Luis; Leighton, Federico
  • Thumbnail Image
    Item
    Large cation-selective pores from rat liver peroxisomal membranes incorporated to planar lipid bilayers
    (1986) Labarca, Pedro; Wolff, Daniel; Soto, Ubaldo; Necochea, Cecilia; Leighton, Federico
    Fusion of a highly purified fraction of rat liver peroxisomal membranes to planar lipid bilayers incorporates large, cation-selective voltage-dependent pores. ThePK/PCl ratio of these pores, estimated in KCl gradients, is close to 4. The pores display several conductance states and spend most of the time open at voltages near 0 mV, closing at more positive and negative voltages. At voltages near 0 mV the most frequent open state has a conductance of 2.4 nS in 0.3m KCl. At voltages more positive and more negative than 10 mV the most frequent open state displays a conductance of 1.2 nS in 0.3m KCl. With these results pore diameters of 3 and 1.5 nm, respectively, can be estimated. We suggest that these pores might account for the unusually high permeability of peroxisomes to low molecular weight solutes. Fusion also incorporates a perfectly anion-selective, two-open states channel with conductances of 50 and 100 pS in 0.1m KCl.
  • Thumbnail Image
    Item
    Mediterranean diet, but not red wine, is associated with beneficial changes in primary haemostasis
    (2003) Mezzano, Diego; Leighton, Federico; Marshall Rivera, Guillermo; Pereira Garcés, Jaime Ignacio
  • Thumbnail Image
    Item
    Methionine sulfoxide reductase A expression is regulated by the DAF-16/FOXO pathway in Caenorhabditis elegans
    (WILEY, 2009) Minniti, Alicia N.; Cataldo, Romina; Trigo, Carla; Vasquez, Luis; Mujica, Patricio; Leighton, Federico; Inestrosa, Nibaldo C.; Aldunate, Rebeca
    P>The methionine sulfoxide reductase system has been implicated in aging and protection against oxidative stress. This conserved system reverses the oxidation of methionine residues within proteins. We analyzed one of the components of this system, the methionine sulfoxide reductase A gene, in Caenorhabditis elegans. We found that the msra-1 gene is expressed in most tissues, particularly in the intestine and the nervous system. Worms carrying a deletion of the msra-1 gene are more sensitive to oxidative stress, show chemotaxis and locomotory defects, and a 30% decrease in median survival. We established that msra-1 expression decreases during aging and is regulated by the DAF-16/FOXO3a transcription factor. The absence of this enzyme decreases median survival and affects oxidative stress resistance of long lived daf-2 worms. A similar effect of MSRA-1 absence in wild-type and daf-2 (where most antioxidant enzymes are activated) backgrounds, suggests that the lack of this member of the methionine repair system cannot be compensated by the general antioxidant response. Moreover, FOXO3a directly activates the human MsrA promoter in a cell culture system, implying that this could be a conserved mechanism of MsrA regulation. Our results suggest that repair of oxidative damage in proteins influences the rate at which tissues age. This repair mechanism, rather than the general decreased of radical oxygen species levels, could be one of the main determinants of organisms' lifespan.
  • Thumbnail Image
    Item
    Nox1-based NADPH oxidase-derived superoxide is required for VSMC activation by advanced glycation end-products
    (2007) San Martín, A.; Foncea Ávila, Rocío Eugenia; Ebensperger González, Roberto Alejandro; Leighton, Federico
  • Thumbnail Image
    Item
    Plant Polyphenol Antioxidants and Oxidative Stress
    (2000) Urquiaga Reus, Inés; Leighton, Federico
  • No Thumbnail Available
    Item
    Polifenoles del Vino y Salud Humana
    (2000) Leighton, Federico
  • No Thumbnail Available
    Item
    Propiedades Antioxidantes del Vino y Sus Componentes
    (1998) Leighton, Federico
  • No Thumbnail Available
    Item
    Validation of self-applicable questionnaire for a Mediterranean dietary index in Chile
    (SOC MEDICA SANTIAGO, 2016) Echeverria, Guadalupe; Urquiaga, Ines; Jose Concha, Maria; Dussaillant, Catalina; Villarroel, Luis; Velasco, Nicolas; Leighton, Federico; Rigotti, Attilio
    Background: Availability of brief dietary indexes that can effectively evaluate dietary patterns and their association with health is critical for prevention and management of several chronic disease conditions. Aim: To adapt a self-applicable Mediterranean Dietary Index in Chile (Chilean-MDI). Material and Methods: The Chilean-MDI was developed based on a previous Mediterranean eating score that was adapted to Chilean dietary habits. This index was further validated in a sample of 153 adults by comparing the concordance between the results obtained by self-application of the Chilean-MDI with those obtained by a trained nutritionist. Additionally, the index was applied in a sample of 53,366 Chilean adults in order to describe the diet quality of our population. Results: There was an adequate concordance between findings obtained by self-application of the Chilean-MDI and those achieved by the nutritionist. The application of the index in Chilean adult population showed abetter diet quality (high Mediterranean diet adherence) among women, with advanced age and among people with higher educational levels. Conclusions: The Chilean-MDI can be successfully self-applied to portray the overall diet quality in the Chilean adult population. Additionally, this dietary index describes overall food intake in Chilean adults, showing demographic trends that are comparable to those obtained with similar indexes applied in other populations.
  • No Thumbnail Available
    Item
  • Thumbnail Image
    Item
    Wine, Diet, Antioxidant Defenses, and Oxidative Damage
    (2002) Pérez, Druso D.; Cuevas, Ada; Leighton, Federico