Browsing by Author "López Alarcón, Camilo Ignacio"
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- Item1,4-Dihydropyridines: Reactivity of nitrosoaryl and nitroaryl derivatives with alkylperoxyl radicals and ABTS radical cation(2004) Valenzuela, V.; Santander, P.; Camargo, C.; Squella, J.A.; López Alarcón, Camilo Ignacio; Nuñez Vergara, L.J.
- ItemA novel and simple ORAC methodology based on the interaction of Pyrogallol Red with peroxyl radicals(2006) López Alarcón, Camilo Ignacio; Lissi, E.
- ItemA Polyvinylpolypyrrolidone (PVPP)-Assisted Folin-Ciocalteu Assay to Assess Total Phenol Content of Commercial Beverages(2014) Bridi, Raquel; Troncoso, M.; Folch Cano, C.; Fuentes, J.; Speisky, Hernán; López Alarcón, Camilo Ignacio
- ItemAnalytical Parameters of the Microplate-Based ORAC-Pyrogallol Red Assay(2011) Ortiz, Rocio; Antilén Lizana, Mónica; Speisky, Hernán; Aliaga Miranda, Margarita Elly; López Alarcón, Camilo Ignacio
- ItemAnti-peroxyl radical quality and antibacterial properties of rooibos infusions and their pure glycosylated polyphenolic constituents(2013) Simpson, Madeline J.; Hjelmqvist, Daisy; López Alarcón, Camilo Ignacio; Karamehmedovic, Nadja; Minehan, Thomas G.; Yepremyan, Akop; Salehani, B.aback; Lissi, Eduardo; Joubert, Elizabeth; Udekwu, Klas I.; Alarcón Abarzúa, Emilio Isaac
- ItemANTIOXIDANT ACTIVITY IN SOUTHERN BRAZIL HYPERICUM SPECIES(2008) Bernardi, A.P.M.; López Alarcón, Camilo Ignacio; Aspee, A.; Rech, S.B.; Von Poser, G.L.; Bridi, Raquel; Dutrafilho C.S.; Lissi, E.
- ItemAntioxidant capacity of herbal infusions and tea extracts: A comparison of ORAC-fluorescein and ORAC-pyrogallol red methodologies(2008) Alarcón Abarzúa, Emilio Isaac; Campos, A.M.; EdwardsM., Ana M; Lissi, E.; López Alarcón, Camilo Ignacio
- ItemAntioxidant capacity of human blood plasma and human urine: Simultaneous evaluation of the ORAC index and ascorbic acid concentration employing pyrogallol red as probe(2008) Torres, P.; Galleguillos, P.; Lissi, E.; López Alarcón, Camilo Ignacio
- ItemAntioxidant capacity of phenolic compounds in acidic medium : a pyrogallol red-based ORAC (oxygen radical absorbance capacity) assay(2013) Atala, Elias; Aspee, Alexis; Speisky, Hernán; Lissi, Eduardo; López Alarcón, Camilo Ignacio
- ItemAntioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles(2010) Romero, Max; Rojano, Benjamin; Mella Raipán, Jaime Alberto; Pessoa Mahana, Carlos David; Lissi, Eduardo; López Alarcón, Camilo Ignacio
- ItemAntioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation(2007) López Alarcón, Camilo Ignacio; Speisky, Hernán; Lissi, Eduardo
- ItemApplication of a Microplate-Based ORAC-Pyrogallol Red Assay for the Estimation of Antioxidant Capacity: First Action 2012.03(2012) Ortiz, Rocio; Antilén Lizana, Mónica; Speisky, Hernán; Aliaga Miranda, Margarita Elly; López Alarcón, Camilo Ignacio; Baugh, Steve
- ItemAscorbic acid contribution to ORAC values in berry extracts: An evaluation by the ORAC-pyrogallol red methodology(2009) Atala Riffo, Elías Duban.; Vásquez Pérez, Luz Mónica; López Alarcón, Camilo Ignacio
- ItemAssessment of the redox cycling properties of nitrocompounds using a superoxide-dependent pyrogallol red bleaching assay(2012) López Alarcón, Camilo Ignacio; Speisky, Hernán; Aliaga Miranda, Margarita Elly; Lissi, E.; Rojas, M.; Faúndez Cáceres, Mario
- ItemAtypical antioxidant activity of non-phenolic amino-coumarins(2018) Zuniga-Nunez, Daniel; Barrias, Pablo; Cardenas-Jiron, Gloria; Soledad Ureta-Zanartu, M.; López Alarcón, Camilo Ignacio; Moran Vieyra, F. Eduardo; Borsarelli, Claudio D.; Alarcon, Emilio I.; Aspee, Alexis
- ItemBinding of rose bengal to lysozyme modulates photooxidation and cross-linking reactions involving tyrosine and tryptophan(2019) Fuentes Lemus, Eduardo Felipe; Mariotti, Michele; Hägglund, Per; Leinisch, Fabian; Fierro Huerta, Angélica; Silva, Eduardo; López Alarcón, Camilo Ignacio; Davies, Michael J.
- ItemChemical Modification of Lysozyme, Glucose 6-Phosphate Dehydrogenase, and Bovine Eye Lens Proteins Induced by Peroxyl Radicals : Role of Oxidizable Amino Acid Residues(2013) Arenas, Andrea; López Alarcón, Camilo Ignacio; Kogan Bocian, Marcelo; Lissi Gervaso, Eduardo A.; Davies, Michael J.; Silva Stevens, Eduardo Andrés
- ItemComparison of different techniques for the preservation and extraction of phlorotannins in the kelp Lessonia spicata (Phaeophyceae): assays of DPPH, ORAC-PGR, and ORAC-FL as testing methods(2016) Cruces, E.; Rojas Lillo, Y.; Ramirez Kushel, E.; Atala, E.; López Alarcón, Camilo Ignacio; Lissi, E.; Gómez, I.
- ItemCompetitive kinetics in free radical reactions of cinnamic acid derivatives. Influence of phenoxyl radicals reactions(2007) López Alarcón, Camilo Ignacio; Aspee, Alexis; Lissi, Eduardo
- ItemComplexation of AAPH (2,2′-azobis(2-methylpropionamidine) dihydrochloride) with cucurbit[7]uril enhances the yield of AAPH-derived radicals(2023) Forero Girón, Angie; Fuentealba Patiño, Denis Alberto; Mariño Ocampo, Nory Johana; Gutiérrez Oliva, Soledad; Herrera Pisani, Bárbara Andrea; Toro Labbé, Alejandro; Fuentes Lemus, Eduardo; Davies, Michael J.; Aliaga Miranda, Margarita Elly; López Alarcón, Camilo IgnacioAAPH (2,2′-azobis(2-methylpropionamidine) dihydrochloride), a water-soluble azo compound is widely employed to produce peroxyl radicals for chemical and biological studies. This compound is shown herein to form a stable inclusion complex with cucurbit[7]uril (CB7), a well-established supramolecular host. Competitive binding assays using berberine dye, isothermal titration calorimetry (ITC), and nuclear magnetic resonance (1H-NMR) experiments, provided evidence for the inclusion of AAPH inside the CB7 cavity with a binding constant of 2.5 ± 0.8 x 105 L mol-1 (determined by ITC). Computational analysis at B3LYP-D3BJ/6-311G(d,p) of the complex (AAPH@CB7) showed interactions of the positively-charged amino groups of AAPH with carbonyl functions at the CB7 entrances. Photolysis of AAPH@CB7 by illumination at 365 nm, gave a higher yield of carbon-centered radicals when compared to the absence of CB7, as evidenced by electron paramagnetic resonance spin trapping using α-phenyl-N-t-butylnitrone. Enhanced radical formation was corroborated by increased consumption of pyrogallol red, free Trp and Trp-containing peptides when these were exposed to AAPH@CB7 in the presence of light. The increased yield of radicals generated by AAPH@CB7 is believed to arise from a pull effect of CB7 portals on initial AAPH-derived carbon-centered radicals generated by photolysis. It is proposed that these radicals are exposed and released to the bulk solution and react with O2 to give peroxyl radicals. These results suggest that the AAPH@CB7 complex can be used to generate high yields of peroxyl radicals for their use in studying these species in material, environmental and biomedical sciences amongst others.