Browsing by Author "Hernández, Sergio"

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    A machine-learning approach for predicting butyrate production by microbial consortia using metabolic network information
    (2025) Silva-Andrade, Claudia; Hernández, Sergio; Saa Higuera, Pedro; Pérez-Rueda, Ernesto; Garrido Cortés, Daniel; Martin, Alberto J.
    Understanding the behavior of microbial consortia is crucial for predicting metabolite production by microorganisms. Genome-scale network reconstructions enable the computation of metabolic interactions and specific associations within microbial consortia underpinning the production of different metabolites. In the context of the human gut, butyrate is a central metabolite produced by bacteria that plays a key role within the gut microbiome impacting human health. Despite its importance, there is a lack of computational methods capable of predicting its production as a function of the consortium composition. Here, we present a novel machine-learning approach leveraging automatically generated genome-scale metabolic models to tackle this limitation. Briefly, all consortia made of two up to 13 members from a pool of 19 bacteria with known genomes, including at least one butyrate producer from a pool of three known producer species, were built and their (maximum) in silico butyrate production simulated. Using network-derived descriptors from each bacteria, butyrate production by the above consortia was used as training data for various machine learning models. The performance of the algorithms was evaluated using k-fold cross-validation and new experimental data, displaying a Pearson correlation coefficient exceeding 0.75 for the predicted and observed butyrate production in two bacteria consortia. While consortia with more than two bacteria showed generally worse predictions, the best machine-learning models still outperformed predictions from genome-scale metabolic models alone. Overall, this approach provides a valuable tool and framework for probing promising butyrate-producing consortia on a large scale, guiding experimentation, and more importantly, predicting metabolic production by consortia.
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    KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover
    (2019) Tapia, Diego; Zamora, Constanza; Espinoza, Javier; Rizzo, Riccardo; González Cárdenas, Alexis; Fuentes Peña, Danitza Natalia; Hernández, Sergio; Cavieres, Viviana A.; Guzmán, Fanny; Arriagada, Gloria; Yuseff Sepúlveda, María Isabel; Mardones, Gonzalo A.; Burgos , Patricia V.; Luini, Alberto; González, Alfonso; Cancino, Jorge; Jiménez, Tomás; Soza Gajardo, Andrea