Browsing by Author "GOREUX, A"

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    INVITRO-PROPAGATION STUDIES OF 3 PROSOPIS SPECIES (PROSOPIS-ALBA, PROSOPIS-CHILENSIS AND PROSOPIS-TAMARUGO) THROUGH SHOOT-TIP CULTURE
    (1985) JORDAN, M; PEDRAZA, J; GOREUX, A
    Plantlets of Prosopis chilensis and P. alba were regenerated in vitro through shoot-tip culture. Shoot-tips of both species initiated growth, showed multiple-shoot formation and developed roots within 4 weeks when cultivated in a nutrient medium containing the phytohormones naphthalene acetic acid (NAA), benzyladenine (BA), and gibberellic acid (GA3), or after subculture using casein hydrolysate and indolebutyric acid (IBA) as single hormone. Under the same conditions the 3-4 uppermost axillary buds of shoot sections set in culture initiated growth and when fragmented and subcultured in presence of IBA regenerated plantlets.
  • No Thumbnail Available
    Item
    POTENTIALITIES OF CELL AND CALLUS-TISSUE CULTURE TO REGENERATE 2 MESQUITE SPECIES (PROSOPIS-TAMARUGO AND PROSOPIS-CHILENSIS)
    (1987) JORDAN, M; CORTES, I; GOREUX, A
    Morphogenetic events in Prosopis tamarugo and P. chilensis calli and cell suspensions were studied in in vitro conditions. Under several growth conditions tested a generalized browning effect in tissues were observed. This effect was partially reduced by the use of cysteine in media. In P. tamarugo, shoot-like structures developed from calli after several subcultures. In P. chilensis, cell suspensions were able to form cell clusters and later on somatic embryoids. At present morphogenetic responses observed in both species occurred in a low frequency.
  • No Thumbnail Available
    Item
    PROMOTION OF ANNONA CHERIMOLA INVITRO SHOOT MORPHOGENESIS AS INFLUENCED BY ANTIOXIDANTS
    (1991) JORDAN, M; ITURRIAGA, L; ROVERARO, C; GOREUX, A
    Nodal sections, internodes, and zygotic embryos of Annona cherimola 'Concha Lisa' were cultivated in vitro in several phytohormone combinations to trigger morphogenesis. De novo multiple shoot formation was the most common response. Various antioxidant compounds, especially PVP, ascorbic acid, and glutathione enhanced this effect and reduced browning, while DIECA and aminooxyacetic acid were less effective in the concentrations tested. Citric acid and cysteine were not beneficial. Combinations of PVP with casein hydrolysate in presence of NAA 0.5 mg/l plus BA 2.0 mg/l were the best blends to promote shoot formation and yielded the highest number of shoots per explant.