Browsing by Author "DIAZ, R"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
- ItemECONOMIC-EVALUATION OF POSTHARVEST LOSSES AND UTILIZATION OF HARD-TO-COOK BEANS - A CASE-STUDY IN CHILE(1991) HOHLBERG, A; AGUILERA, JM; DIAZ, R
- ItemLARGE V-WAVES IN THE PULMONARY CAPILLARY WEDGE PRESSURE TRACING WITHOUT MITRAL REGURGITATION - THE INFLUENCE OF THE PRESSURE VOLUME RELATIONSHIP ON THE V-WAVE SIZE(1983) PICHARD, AD; DIAZ, R; MARCHANT, E; CASANEGRA, P
- ItemPENICILLIUM-PURPUROGENUM PRODUCES SEVERAL XYLANASES - PURIFICATION AND PROPERTIES OF 2 OF THE ENZYMES(1995) BELANCIC, A; SCARPA, J; PEIRANO, A; DIAZ, R; STEINER, J; EYZAGUIRRE, JThe fungus Penicillium purpurogenum produces several extracellular xylanases. The two major forms (xylanases A and B) have been purified and characterized. After ammonium sulfate precipitation and chromatography in Bio-Gel P 100, xylanase A was further purified by means of DEAE-cellulose, hydroxylapatite and CM-Sephadex, and xylanase B by DEAE-cellulose and CM-Sephadex. Both xylanases showed apparent homogeneity in SDS-polyacrylamide gel electrophoresis. Xylanase A (33 kDa) has an isoelectric point of 8.6, while xylanase B (23 kDa) is isoelectric at pH 5.9. Antisera against both enzymes do not cross-react. The amino terminal sequences of xylanases A and B show no homology. The results obtained suggest that the enzymes are produced by separate genes and they may perform different functions in xylan degradation.