Browsing by Author "Zarate, Rafaella, V"

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    Optimization of the Light-On system in a lentiviral platform to a light-controlled expression of genes in neurons
    (2021) Zarate, Rafaella, V; Arancibia, Duxan; Fernandez, Anllely; Signorelli, Janetti R.; Larrondo, Luis F.; Estela Andres, Maria; Zamorano, Pedro
    Background: Molecular brain therapies require the development of molecular switches to control gene expression in a limited and regulated manner in time and space. Light-switchable gene systems allow precise control of gene expression with an enhanced spatio-temporal resolution compared to chemical inducers. In this work, we adapted the existing light-switchable Light-On system into a lentiviral platform, which consists of two modules: (i) one for the expression of the blue light-switchable transactivator GAVPO and (ii) a second module containing an inducible-UAS promoter (UAS) modulated by a light-activated GAVPO.