Browsing by Author "YAMADA, Y"

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    LIPOPOLYSACCHARIDES OF THIOBACILLUS SPECIES CONTAINING LIPID-A WITH 2,3-DIAMINO-2,3-DIDEOXYGLUCOSE
    (1987) YOKOTA, A; RODRIGUEZ, M; YAMADA, Y; IMAI, K; BOROWIAK, D; MAYER, H
    Lipopolysaccharides were isolated from two strains of Thinobacillus ferrooxidans and one strain each of Thiobacillus thiooxidans, Thiobacillus novellus and Thiobacillus sp. IFO 14570. Neutral sugars, 2-keto-3-deoxyoctonate, fatty acids and the rare 2,3-diamino-2,3-dideoxyglucose were detected in all lipopolysaccharides. Lipopolysaccharides of both T. ferrooxidans strains contained L-glycero-D-manno-heptose, whereas that of T. thiooxidans contained both L-glycero-D-manno-heptose and D-glycero-D-manno-heptose. On the other hand, heptoses were absent in lipopolysaccharides of T. novellus and Thiobacillus sp. IFO 14570. Lipid A of T. ferrooxidans and T. thiooxidans contained both glucosamine and 2,3-diamino-2,3-dideoxyglucose, in contrast, lipid A of T. novellus and the Thiobacillus sp. IFO 14570 most likely contain only 2,3-diamino-2,3-dideoxyglucose as backbone sugar. Deoxycholate polyacrylamide gel electrophoresis revealed S-type character for all lipopolysaccharides studied. The significance of the lipopolysccharide composition for taxonomic and phylogenetic questions with regard to thiobacilli is discussed.