Browsing by Author "Villalón, Manuel J."
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- Item17-β-Estradiol upregulates COX-2 in the rat oviduct(2006) Pérez, S.; Villalón, Manuel J.
- Item2-Methoxyestradiol inhibits progesterone-dependent tissue factor expression and activity in breast cancer cells(2010) Quezada, M.; Diaz, J.; Henriquez, S.; Bravo Castillo, María Loreto; Aranda, E.; Oliva, B.; Villalón, Manuel J.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Brosens, J. J.; Lange, C. A.; Owen, Gareth Ivor2-Methoxyestradiol (2ME) is an endogenous metabolite of 17β-estradiol with antiangiogenic and antitumor properties, although its mechanisms of action remain unclear. Progestins in hormone replacement therapy increase the risk of breast cancer. Progesteron
- ItemA non-genomic signaling pathway shut down by mating changes the estradiol-induced gene expression profile in the rat oviduct(2010) Parada Bustamante, Alexis Andrés.; Rios Raggio, Mariana; Villalón, Manuel J.
- ItemAcidic pH and Increasing [Ca2+] Reduce The Swelling of Mucins in Primary Cultures of Human Cervical Cells(2002) Espinosa, M.; Villalón, Manuel J.
- ItemATP and adenosine trigger the interaction of plasma membrane IP3 receptors with protein kinase A in oviductal ciliated cells(2007) Barrera Rojas, Nelson Patricio; Villalón, Manuel J.
- ItemCalcium (Ca2+) waves data calibration and analysis using image processing techniques(2013) Milovic Fabregat, Carlos Andrés; Oses, Carolina; Villalón, Manuel J.; Uribe Arancibia, Sergio A.; Prieto Vásquez, Claudia; Andía Kohnenkampf, Marcelo Edgardo; Irarrázaval Mena, Pablo; Tejos Núñez, Cristián Andrés; Lizama, CarlosAbstract Background Calcium (Ca2+) propagates within tissues serving as an important information carrier. In particular, cilia beat frequency in oviduct cells is partially regulated by Ca2+ changes. Thus, measuring the calcium density and characterizing the traveling wave plays a key role in understanding biological phenomena. However, current methods to measure propagation velocities and other wave characteristics involve several manual or time-consuming procedures. This limits the amount of information that can be extracted, and the statistical quality of the analysis. Results Our work provides a framework based on image processing procedures that enables a fast, automatic and robust characterization of data from two-filter fluorescence Ca2+ experiments. We calculate the mean velocity of the wave-front, and use theoretical models to extract meaningful parameters like wave amplitude, decay rate and time of excitation. Conclusions Measurements done by different operators showed a high degree of reproducibility. This framework is also extended to a single filter fluorescence experiments, allowing higher sampling rates, and thus an increased accuracy in velocity measurements.
- ItemCharacterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells(2008) Kato Cardemil, Sumie Rode; Villalón, Manuel J.; Cuello F., Mauricio; Owen, Gareth Ivor; Espinoza, Natalia; Lange, Soledad
- ItemCharacterization of a Marker for Tracheal Basal Cells(1995) Aitken, ML.; Villalón, Manuel J.
- ItemCharacterization of a Marker of Differentiation for Tracheal Ciliated Cells Independent of Ciliation(1993) Aitken, ML.; Villalón, Manuel J.
- ItemClass of Skew-Distributions: Theory and Applications in Biology(2006) Barrera Rojas, Nelson Patricio; Villalón, Manuel J.
- ItemControl of basal ciliary activity and response to mechanical and chemical stimuli depend on extracellular ATP through an autocrine/paracrine mechanism.(2014) Droguett Quezada, Karla Denise; Villalón, Manuel J.; Pontificia Universidad Católica de Chile. Facultad de Ciencias BiológicasEn el epitelio mucociliado de las vías respiratorias y del oviducto, la frecuencia de batido ciliar (CBF) es el principal factor que determina la efectividad del transporte mucociliar. CBF se puede medir en un estado basal o incrementada por un estimulo químico o mecánico. ATP es un conocido estimulante de la CBF, efecto que es mediado por la activación de receptores purinérgicos. El incremento en la CBF inducido por ATP depende de la liberación de Ca2+ de almacenes intracelulares y de la entrada de Ca2+ desde el medio extracelular. En células del epitelio respiratorio, ATP es liberado al medio extracelular de manera constitutiva o en respuesta a una estimulación mecánica (MS), por un mecanismo que involucraría hemicanales (HCs) de panexina 1 (Panx1) o de conexina 43 (Cx43). El objetivo de este estudio fue determinar si ATP liberado por el epitelio mucociliado contribuye en el mecanismo de control de la actividad ciliar basal y si el incremento de la CBF inducido por una estimulación química y mecánica es mediado por la liberación de ATP.
- ItemControl of Ciliary Movement in Mamalian Oviductal Ciliated Cells(1991) Verdugo, P.; Villalón, Manuel J.
- ItemCultivo primario de células ciliadas de adenoides humanos. Un modelo experimental para evaluar la actividad ciliar in vitro(2007) González, Claudia.; Sánchez Dueñas, María Trinidad; Villalón, Manuel J.
- ItemEffect of Nitric Oxide Synthase Inhibitors on Ovum Transport and Oviductal Smooth Muscle Activity in the Rat Oviduct(2000) Pérez, S.; Villalón, Manuel J.
- ItemEpidermal Growth Factor Prevents Prepartum Luteolysis in the Rat(2005) Ribeiro, M.; Villalón, Manuel J.
- ItemEpithelial Cell Culture from Human Adenoids : A Functional Study Model for Ciliated and Secretory Cells(2013) González, Claudia; Espinosa de la Fuente, Marisol; Sánchez Dueñas, María Trinidad; Droguett, Karla; Ríos Raggio, Mariana; Fonseca Arrieta, María Ximena; Villalón, Manuel J.
- ItemFunctional Cross Talk After Activation of P2 and P1 Receptors in Oviductal Ciliated Cells(2000) Morales, B.; Villalón, Manuel J.
- ItemIdentification of Gap Junctions in the Oviduct and Regulation of Connexins During Development and Sexual Hormones(1997) Hermoso, M.; Sáez, Juan Carlos; Villalón, Manuel J.Gap junctions in epithelial cells of the oviduct were identified by immunohistochemistry and Western blotting, Immunohistochemical studies showed that rat, hamster, mouse, and human oviducts contained connexin26 and connexin43 but not connexin32, The content of both connexins in the oviduct depended on the cell type, state of maturation and hormone status, During ontogeny, the epithelial and smooth muscle cells of immature rat oviducts (< 30 days) contained a low amount of connexin43, and connexin26 was undetectable, In mature oviducts (> 30 days), however, connexin26 was detected only in the isthmus and in localized regions of the ampullar epithelial layer, Moreover, at this age, the amount of connexin43 was high in both cell strata throughout the entire organ, During the estrous cycle, levels of connexin43 in the isthmic but not in the ampullar segment were higher in the proestrous and estrous day than at diestrous days, In addition, estrogen treatment produced a significant increase in total and phosphorylated isoforms of connexin43 Levels in oviducts of pregnant rats, The estrogen effect was prevented by the simultaneous administration of progesterone which by itself did not affect the levels of connexin43. The high content of connexins found between oviductal cells as well as their responsiveness to hormone regulation, suggest that gap junctions might be involved in coordinating oviductal cell functions such as smooth muscle contraction and epithelial ciliary beat.
- ItemIn vivo postprandial bioavailability of interesterified-lipids in sodium-caseinate or chitosan based O/W emulsions(2015) Farfán Martínez, Mariel; Villalón, Manuel J.; Ortíz Scarlazetta, María Elena; Bouchon Aguirre, Pedro Alejandro