Browsing by Author "Villagra, E"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Complete sequence of the genome of the human isolate of Andes virus CHI-7913
    (2003) Tischler, ND; Fernández, J; Müller, I; Martínez, R; Galeno, H; Villagra, E; Mora, J; Ramírez, E; Rosemblatt, M; Valenzuela, PDT
    We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M. and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed.
  • Thumbnail Image
    Item
    Isolation of Coxsackie B-5 virus from a patient with a fatal meningoencephalitis
    (SOC MEDICA SANTIAGO, 1995) delaFuente, G; Palacios, O; Villagra, E; Villanueva, ME
    A nine months old boy was admitted to the hospital with the diagnosis of meningoencephalitis 15 days after having a clinically diagnosed chickenpox. Lumbar puncture showed clear CSF with 0.23 g/l of proteins, 57 mg/dl of glucose, 30 red cells/mm(3) and 5 leukocytes/mm(3). Blood count showed a packed red cell volume of 22%, a hemoglobin of 7 g/dl, 14800 leukocytes with 1% eosinophils, 5% band and 39% segmented neutrophils, 50% lymphocytes and 5% monocytes and a decreased platelet count. On the fourth hospitalization day, the patient had vomiting, irritability and stiff neck. A new lumbar puncture showed a clear CSF that differed from the former only in the glucose level that increased to 102 mg/dl. The patient died and the necropsy showed a congestive and enlarged brain and congestive meninges infiltrated with lymphocytes. There was lymphoid follicle hyperplasia in the small bowel and enlarged mesenteric lymph nodes. samples of brain, brain stem, spinal cord and stools were sent for virological study. A coxsackie B-5 virus was isolated from the spinal cord sample.