Browsing by Author "Vicuna, R"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Characterization of a bacterial consortium degrading the lignin model compound vanillyl-beta-D-glucopyranoside
    (1997) Cespedes, R; Gonzalez, B; Vicuna, R
    Aryl-beta-D-glycosides have been described as intermediates in lignin biodegradation. The catabolism of this kind of compounds has been poorly studied. In this work, the isolation and initial characterization of a bacterial consortium degrading vanillyl-beta-D-glucopyranoside is reported. The consortium is able to grow on and completely degrade the dimer vanillyl-beta-D-glucopyranoside. The consortium is composed of three microorganisms: strain 2.1 is closely related to Chryseomonas luteola; strain 2.2, identified as Moraxella pheylpyruvica and strain 2.3, preliminary identified as Pseudomonas spp. Complete degradation of the glycoside required strains 2.1 and 2.2, although only strain 2.1 was able to cleave the aryl-glycoside linkage. Oxidation of vanillyl alcohol, one product of the aryl-glycoside cleaving reaction, to the respective acid was carried out by strain 2.2. Vanillic acid was used as growth substrate by strains 2.2 and 2.3. Strain 2.1 can grow on other aryl-P-glycosides, accumulating the aryl moiety in the culture. P-Glucosidase present in strain 2.1 is probably the key enzyme in the catabolism of aryl-glycosides by this consortium.
  • No Thumbnail Available
    Item
    lip-like genes in Phanerochaete sordida, and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity
    (1996) Rajakumar, S; Gaskell, J; Cullen, D; Lobos, S; Karahanian, E; Vicuna, R
    Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was amplified from P. sordida RNA. In contrast, no evidence was obtained for transcription of C. subvermispora LiP genes.