Browsing by Author "Veloso-Gimenez, Valentina"

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    Oxygenation by Intravascular Photosynthesis Reduces Kidney Damage During ex Vivo Preservation
    (2024) Veloso-Gimenez, Valentina; Cardenas-Calderon, Camila; Castillo, Valentina; Carvajal, Felipe; Gallardo-Aguero, Daniela; Gonzalez-Itier, Sergio; Corrales-Orovio, Rocio; Becerra, Daniela; Miranda, Miguel; Rebolledo, Rolando; San Martin, Sebastian; Boric, Mauricio P.; Egana, Jose Tomas
    Several clinical issues are associated with reduced oxygen delivery to tissues due to impaired vascular perfusion; moreover, organs procured for transplantation are subjected to severe hypoxia during preservation. Consequently, alternative tissue oxygenation is an active field in biomedical research where several innovative approaches have been recently proposed. Among these, intravascular photosynthesis represents a promising approach as it relies on the intrinsic capacity of certain microorganisms to produce oxygen upon illumination. In this context, this work aims at the development of photosynthetic perfusable solutions that could be applied to preserve organs for transplantation purposes. Our findings demonstrate that a biocompatible physiological solution containing the photosynthetic microalgae Chlamydomonas reinhardtii can fulfill the metabolic oxygen demand of rat kidney slices in vitro. Furthermore, intravascular administration of this solution does not induce tissue damage in the rat kidneys. Moreover, kidney slices obtained from these algae-perfused organs exhibited significantly improved preservation after 24 h of incubation in hypoxia while exposed to light, resulting in reduced tissue damage and enhanced metabolic status. Overall, the results presented here contribute to the development of alternative strategies for tissue oxygenation, supporting the use of perfusable photosynthetic solutions for organ preservation in transplantation.