Browsing by Author "VALDES, F"
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- ItemDEOXYRIBONUCLEIC-ACID POLYMERASE FROM THE MARINE PSEUDOMONAS SP BAL-31(1980) VICUNA, R; VALDES, F; MEDINA, A; YUDELEVICH, AA DNA-dependent DNA polymerase (DNA nucleotidyltransferase) was purified 3000-fold from the marine Pseudomonas sp. BAL-31. The MW of the native enzyme was estimated by glycerol gradient sedimentation to be 110,000. The enzyme migrated in sodium dodecyl sulfate-acrylamide gels as a single polypeptide with a MW of 105,000. An absolute requirement for divalent cation was satisfied by Mg2+ or Mn2+ at concentrations of 1 mM. Monovalent cations at concentrations higher than 50 mM showed an inhibitory effect. The polymerase activity was resistant to N-ethylmaleimide and showed a wide pH optimum.
- ItemRAPID PROCEDURE FOR PURIFYING A RESTRICTION ENDONUCLEASE FROM THERMUS-THERMOPHILUS (TTH I)(1980) VENEGAS, A; VICUNA, R; ALONSO, A; VALDES, F; YUDELEVICH, A