Browsing by Author "Toro, Guillermo"

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    Integration of lipidomics and transcriptomics provides new insights into lipid metabolism in response to water deficit in Prunus spp. rootstock leaves
    (Elsevier B.V., 2025) Olmedo, Patricio; Nunez Lillo, Gerardo; Toro, Guillermo; Opazo, Ismael; Salvatierra, Ariel; Meneses Araya, Claudio Antonio; Pedreschi, Romina; Pimentel, Paula
    The mechanisms underlying the role of lipids in the response to water deficit in Prunus species have not yet been elucidated. To investigate these, a drought-tolerant rootstock (R40) and a drought-sensitive rootstock (R20) were exposed to well-watered (WW) and water deficit (WD) conditions. We combined physiological, lipidomics, and transcriptomics analyses to elucidate lipid dynamics in rootstock leaves and roots when coping drought. Data showed that R40 genotype possessed a higher stomatal conductance and photosynthetic rate under WD conditions. Lipidomic profiling indicated that most of differences were found in leaves between both genotypes. Under WD conditions, R40 genotype showed a higher number of lipids accumulated, such as ceramides, unsaturated fatty acids, and triacylglycerols. Also, when comparing WW and WD conditions, we observed that drought induced major changes in the R20 genotype. Interestingly, WD reduced the number of accumulated compounds, suggesting a lipid remodeling associated with degradation. Transcriptomic analysis of lipid-related genes showed that the R20 genotype were more responsive to WD, decreasing the expression of these transcripts. A decrease in fatty acid biosynthesis and desaturation was induced in the R20 genotype under WD, while the R40 genotype showed an increased expression of genes associated mainly with biosynthesis of fatty acids and triacylglycerol.
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    Plasticity of the Root System Architecture and Leaf Gas Exchange Parameters Are Important for Maintaining Bottle Gourd Responses under Water Deficit
    (2020) Zacarias Rafael, Dinoclaudio; Arriagada, Osvin; Toro, Guillermo; Mashilo, Jacob; Mora-Poblete, Freddy; Contreras-Soto, Rodrigo Ivan
    The evaluation of root system architecture (RSA) development and the physiological responses of crop plants grown under water-limited conditions are of great importance. The purpose of this study was to examine the short-term variation of the morphological and physiological plasticity of Lagenaria siceraria genotypes under water deficit, evaluating the changes in the relationship between the root system architecture and leaf physiological responses. Bottle gourd genotypes were grown in rhizoboxes under well-watered and water deficit conditions. Significant genotype-water regime interactions were observed for several RSA traits and physiological parameters. Biplot analyses confirmed that the drought-tolerant genotypes (BG-48 and GC) showed a high net CO2 assimilation rate, stomatal conductance, transpiration rates with a smaller length, and a reduced root length density of second-order lateral roots, whereas the genotypes BG-67 and Osorno were identified as drought-sensitive and showed greater values for average root length and the density of second-order lateral roots. Consequently, a reduced length and density of lateral roots in bottle gourd should constitute a response to water deficit. The root traits studied here can be used to evaluate bottle gourd performance under novel water management strategies and as criteria for breeding selection.