Browsing by Author "Tapia, P."

Now showing 1 - 9 of 9
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    Catastrophic respiratory failure from tuberculosis pneumonia: Survival after prolonged extracorporeal membrane oxygenation support
    (2013) Andresen Hernández, Max; Tapia, P.; Mercado, M.; Bugedo Tarraza, Guillermo; Bravo, S.; Regueira Heskia, Tomás
    Tuberculosis (TB) is an uncommon cause of severe respiratory failure, even in highly endemic regions. Mortality in cases requiring mechanical ventilation (MV) varies between 60 and 90%. The use of extracorporeal membrane oxygenation (ECMO) is not frequently needed in TB. We report the case of a 24 year old woman diagnosed with bilateral pneumonia that required MV and intensive care, patient was managed with prone ventilation for 48 h, but persisted in refractory hypoxemia. Etiological study was only positive for mycobacterium tuberculosis. As a rescue therapy arterio-venous extracorporeal CO2 removal was started and lased for 4 days, but fails to support the patient due to greater impairment of oxygenation. Veno-venous ECMO was then initiated, thus normalizes gas exchanged and allows lungs to rest. ECMO was maintained for 36 days, with two episodes of serious complication treated successfully. Given the absence of clinical improvement and the lack of nosocomial infection, at 42-day of ICU stay methylprednisolone 250 mg daily for 4 days was started, since secondary organizing pneumonia associated with TB was suspected. Thereafter progressive improvement in pulmonary mechanics and reduction of pulmonary opacities was observed, allowing the final withdrawal of ECMO. Percutaneous tracheostomy was performed and the patient remained connected until her transfer to her base hospital at day 59 of admission to our unit. The tracheostomy was removed prior to hospital discharge, and the patient is today at home. Prolonged ECMO support is a useful and potentially successful tool in catastrophic respiratory failure caused by TB.
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    Células prostáticas circulantes para la detección de cáncer
    (2016) Murray, N. P.; Tapia, P.; Fuentealba, C.; Orellana, N.; Dueñas, R.
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    Chest physiotherapy is not clinically indicated for infants receiving outpatient care for acute wheezing episodes
    (2014) Castro-Rodriguez, J. A.; Silva, R.; Tapia, P.; Salinas, P.; Tellez, Alvaro; Leisewitz, T.; Sánchez Díaz, Ignacio
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    Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
    (2023) Figueroa, A.-M.; Stolzenbach, F.; Tapia, P.; Cortés, V.
    Brown adipose tissue (BAT) is only present in mammals and has a thermogenic function. Brown adipocytes are characterized by a multilocular cytoplasm with multiple lipid droplets, a central nucleus, a high mitochondrial content, and the expression of uncoupling protein 1 (UCP1). BAT has been proposed as a potential therapeutic target for obesity and its associated metabolic disorders due to its ability to dissipate metabolic energy as heat. To investigate BAT function and regulation, brown adipocyte culturing is indispensable. The present protocol optimizes tissue processing and cell differentiation for culturing brown adipocytes from newborn mice. Additionally, procedures for the imaging of differentiated adipocytes with both confocal immunofluorescence and transmission electron microscopy are shown. In the brown adipocytes differentiated with the techniques described herein, the major defining features of classical BAT are preserved, including high UCP1 levels, increased mitochondrial mass, and very close physical contact between the lipid droplets and mitochondria, making this method a valuable tool for BAT studies.
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    Extracorporeal membrane oxygenation improves survival in a novel 24-hour pig model of severe acute respiratory distress syndrome
    (2016) Araos, J.; Alegría Aguirre, Luz Katiushka; Garcia, P.; Damiani Rebolledo, L. Felipe; Tapia, P.; Soto, D.; Salomon, T.; Retamal Montes, Jaime; Bugedo Tarraza, Guillermo; Bruhn, Alejandro; Rodriguez, F.; Amthauer, M.; Erranz, B.; Castro, G.; Carreno, P.; Medina, T.; Cruces, P.
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    Method Based on the beta-Lactamase PenPC Fluorescent Labeled for beta-Lactam Antibiotic Quantification in Human Plasma
    (2016) Andresen Hernández, Max; Wong, K.; Leung, Y.; Wong, W.; Chan, P.; Andresen Vásquez, Max; Alegría Aguirre, Luz Katiushka; Silva, C.; Tapia, P.; Downey Concha, Patricio; Soto, D.
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    Occurrence, source estimation, and risk assessment of Polycyclic Aromatic Hydrocarbons in coastal seawaters from the Quintero Industrial Complex (Valparaiso, Chile)
    (2023) Galban-Mmalagon, C. J.; Zapata, J.; Perez-Venegas, D. J.; Vargas, R.; Latorre-Padilla, N.; Luarte, T.; Ahrendt, C.; Hirmas-Olivares, A.; Gomez-Aburto, V.; Tapia, P.; Isamit, V.; Arce, P.; Sanchez, C.; Pozo, K.
    In the 1960s, the Quintero industrial complex was inaugurated in Chile. This began a history of dramatic anthropo-genic impacts on the Chilean coast. Among the known, we could mention high atmospheric emissions of chemicals due to combustion processes and frequent oil spills. For this reason, we surveyed the concentrations of fifteen EPAPAHs in the surface coastal waters of the Quintero Bay area in 2015. The levels found are in the range of the highest levels when reviewing the literature (0.97 mu g L-1 up to 9.84 mu g L-1). The highest levels were found in the vicinity of the industrial complex and decreased in the other two zones. The concentration of individual compounds significantly ex-ceeds the levels recommended by the EPA (Environmental Protection Agency) and the EU water framework directive (WFD). The risk estimations revealed that PAH concentrations represent high-risk for wildlife. Molecular ratios of PAHs were used to identify the possible sources, being these were mainly of pyrogenic origin, agreeing with an origin in the combustion of wood, coal, grass, and fossil fuels. This study contributes to the first data for surface water in a country's highly impacted industrial coastal area.
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    Redefining micrometastasis in prostate cancer - a comparison of circulating prostate cells, bone marrow disseminated tumor cells and micrometastasis: Implications in determining local or systemic treatment for biochemical failure after radical prostatectomy
    (2012) Murray, N. P.; Reyes, E.; Tapia, P.; Badinez, L.; Orellana, N.; Fuentealba, C.; Olivares, R.; Porcell, J.; Duenas, R.
    The presence of cells positive for cytokeratins or prostate-specific antigen (PSA) in bone marrow aspirates (BMAs) has been used to indicate the presence of micrometastasis. The aim of this prospective study of prostate cancer patients was to determine the presence of prostate cells in blood and BMAs and to compare them with bone marrow biopsy touch prep samples. The results indicated that there was a satisfactory concordance between circulating prostate cells (CPCs) in blood and disseminated tumor cells (DTCs) in BMAs for all Gleason scores (kappa>0.50). However, neither were concordant with the presence of prostate cells in bone marrow biopsies except for high-grade tumors, Gleason 8 and 9. Phenotypic characteristics of CPCs and DTCs were identical (kappa>0.9) but were different than cells detected in bone marrow biopsies (kappa<0.2). The expression of matrix metalloproteinase-2 (MMP-2) in bone marrow biopsies was positively associated with the Gleason score (trend Chi-squared <0.05) and may explain the differences between the presence of DTCs and the presence of prostate cells in bone marrow biopsies. If the presence of DTCs was used to indicate micrometastatic disease, 20% of patients would be misclassified compared to micrometastasis defined as patients with a positive biopsy. This may have clinical implications for patients with low-grade tumors.