Browsing by Author "Takakura, Ana C."

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    Ablation of brainstem C1 neurons improves cardiac function in volume overload heart failure
    (2019) Andrade Andrade, David Cristóbal; Toledo, Camilo; Díaz, Hugo S.; Lucero, Claudia; Arce Álvarez, Alexis; Oliveira, Luis M.; Takakura, Ana C.; Moreira, Thiago S.; Schultz, Harold D.; Del Rio, Rodrigo; Marcus, Noah J.; Alcayaga Urbina, Julio Andrés
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    Cardiorespiratory alterations following intermittent photostimulation of RVLM C1 neurons: Implications for long-term blood pressure, breathing and sleep regulation in freely moving rats
    (2022) Toledo, Camilo; Andrade, David C.; Diaz-Jara, Esteban; Ortolani, Domiziana; Bernal-Santander, Ignacio; Schwarz, Karla G.; Ortiz, Fernando C.; Marcus, Noah J.; Oliveira, Luiz M.; Takakura, Ana C.; Moreira, Thiago S.; Del Rio, Rodrigo
    Aim Sympathoexcitation and sleep-disordered breathing are common contributors for disease progression. Catecholaminergic neurons from the rostral ventrolateral medulla (RVLM-C1) modulate sympathetic outflow and have anatomical projections to respiratory neurons; however, the contribution of highly selective activation of RVLM-C1 neurons on long-term autonomic and breathing (dys)regulation remains to be understood. Methods To explore this relationship, a lentiviral vector carrying the light-sensitive cation channel channelrhodopsin-2 (LVV-PRSX8-ChR2-YFP) was unilaterally injected into the RVLM of healthy rats. On the contralateral side, LVV-PRSX8-ChR2-YFP was co-injected with a specific immunotoxin (D beta H-SAP) targeted to eliminate C1 neurons. Results Intermittent photostimulation of RVLM-C1 in vivo, in unrestrained freely moving rats, elicited long-term facilitation of the sympathetic drive, a rise in blood pressure and sympatho-respiratory coupling. In addition, photoactivation of RVLM-C1 induced long-lasting ventilatory instability, characterized by oscillations in tidal volume and increased breathing variability, but only during non-rapid eye movement sleep. These effects were not observed when photostimulation of the RVLM was performed in the presence of D beta H-SAP toxin. Conclusions The finding that intermittent activation of RVLM-C1 neurons induces autonomic and breathing dysfunction suggest that episodic stimulation of RVLM-C1 may serve as a pathological substrate for the long-term development of cardiorespiratory disorders.
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    Rostral ventrolateral medullary catecholaminergic neurones mediate irregular breathing pattern in volume overload heart failure rats
    (2019) Toledo, Camilo; Andrade, David C.; Diaz, Hugo S.; Pereyra, Katherin V.; Schwarz, Karla G.; Diaz-Jara, Esteban; Oliveira, Luiz M.; Takakura, Ana C.; Moreira, Thiago S.; Schultz, Harold D.; Marcus, Noah J.; Del Rio, Rodrigo
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