Browsing by Author "Tabary, Mohammadreza"

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    Oxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury
    (2024) Suber, Tomeka L.; Tabary, Mohammadreza; Bain, William; Olonisakin, Tolani; Lockwood, Karina; Xiong, Zeyu; Zhang, Yingze; Kohli, Naina; Furguiele, Lauren; Peñaloza Cerda, Hernán Felipe; Mcverry, Bryan J.; Rose, Jason J.; Shah, Faraaz; Methe, Barbara; Li, Kelvin; Mallampalli, Rama K.; Chen, Kong; Fan, Li; Morris, Alison; Tyurin, Vladimir A.; Samovich, Svetlana N.; Bayir, Hulya; Tyurina, Yulia Y.; Kagan, Valerian; Lee, Janet S.
    E-cigarette/vaping-associated lung injury (EVALI) is strongly associated with vitamin E acetate and often occurs with concomitant tetrahydrocannabinol (THC) use. To uncover pathways associated with EVALI, we examined cytokines, transcriptomic signatures, and lipidomic profiles in bronchoalveolar lavage fluid (BALF) from THC-EVALI patients. At a single center, we prospectively enrolled mechanically ventilated patients with EVALI from THC-containing products (N = 4) and patients with non-vaping acute lung injury and airway controls (N = 5). BALF samples were analyzed by Luminex multiplex assay, RNA sequencing, and mass spectrometry. After treating BEAS-2B lung epithelial cells with vaping and non-vaping BALF, LDH release was quantified. THC-EVALI BALF had significant increases in IFN gamma, CCL2, CXCL5, and MMP2 relative to non-vaping patients. RNA sequencing showed enrichment for biological oxidation, glucuronidation, and fatty acid metabolism pathways. Oleic acid and arachidonic acid metabolites were increased in THC-EVALI, as were oxidized phosphatidylethanolamines (PE) such as PE(38:4). THC-EVALI BALF induced more LDH release compared to BALF from non-vaping patients. Thus, THC-EVALI is characterized by altered phospholipid composition, accumulation of lipid oxidation products, and increased pro-inflammatory mediators that may contribute to epithelial cell death. These findings serve as a framework to study novel oxidized phospholipids implicated in the pathogenesis of EVALI.
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    Oxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury
    (NATURE PORTFOLIO, 2024) Suber, Tomeka L.; Tabary, Mohammadreza; Bain, William; Olonisakin, Tolani; Lockwood, Karina; Xiong, Zeyu; Zhang, Yingze; Kohli, Naina; Furguiele, Lauren; Peñaloza Cerda, Hernán Felipe; Mcverry, Bryan J.; Rose, Jason J.; Shah, Faraaz; Methe, Barbara; Li, Kelvin; Mallampalli, Rama K.; Chen, Kong; Fan, Li; Morris, Alison; Tyurin, Vladimir A.; Samovich, Svetlana N.; Bayir, Hulya; Tyurina, Yulia Y.; Kagan, Valerian; Lee, Janet S.
    E-cigarette/vaping-associated lung injury (EVALI) is strongly associated with vitamin E acetate and often occurs with concomitant tetrahydrocannabinol (THC) use. To uncover pathways associated with EVALI, we examined cytokines, transcriptomic signatures, and lipidomic profiles in bronchoalveolar lavage fluid (BALF) from THC-EVALI patients. At a single center, we prospectively enrolled mechanically ventilated patients with EVALI from THC-containing products (N = 4) and patients with non-vaping acute lung injury and airway controls (N = 5). BALF samples were analyzed by Luminex multiplex assay, RNA sequencing, and mass spectrometry. After treating BEAS-2B lung epithelial cells with vaping and non-vaping BALF, LDH release was quantified. THC-EVALI BALF had significant increases in IFN gamma, CCL2, CXCL5, and MMP2 relative to non-vaping patients. RNA sequencing showed enrichment for biological oxidation, glucuronidation, and fatty acid metabolism pathways. Oleic acid and arachidonic acid metabolites were increased in THC-EVALI, as were oxidized phosphatidylethanolamines (PE) such as PE(38:4). THC-EVALI BALF induced more LDH release compared to BALF from non-vaping patients. Thus, THC-EVALI is characterized by altered phospholipid composition, accumulation of lipid oxidation products, and increased pro-inflammatory mediators that may contribute to epithelial cell death. These findings serve as a framework to study novel oxidized phospholipids implicated in the pathogenesis of EVALI.
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    The matricellular protein thrombospondin-1 in lung inflammation and injury
    (2022) Tabary, Mohammadreza; Gheware, Atish; Peñaloza Cerda, Hernán F.; Lee, Janet S.
    Matricellular proteins comprise a diverse group of molecular entities secreted into the extracellular space. They interact with the extracellular matrix (ECM), integrins, and other cell-surface receptors, and can alter matrix strength, cell attachment to the matrix, and cell-cell adhesion. A founding member of this group is thrombospondin-1 (TSP-1), a high molecular-mass homotrimeric glycoprotein. Given the importance of the matrix and ECM remodeling in the lung following injury, TSP-1 has been implicated in a number of lung pathologies. This review examines the role of TSP-1 as a damage controller in the context of lung inflammation, injury resolution, and repair in noninfectious and infectious models. This review also discusses the potential role of TSP-1 in human diseases as it relates to lung inflammation and injury.