Browsing by Author "Suchy, FJ"

Now showing 1 - 3 of 3
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    Bile secretory function in the obese Zucker rat: evidence of cholestasis and altered canalicular transport function
    (BMJ PUBLISHING GROUP, 2004) Pizarro, M; Balasubramaniyan, N; Solis, N; Solar, A; Duarte, I; Miquel, JF; Suchy, FJ; Trauner, M; Accatino, L; Ananthanarayanan, M; Arrese, M
    Background: Obese Zucker rats (ZR) have been used as an experimental model for non-alcoholic fatty liver disease and are particularly susceptible to various types of liver injury. Bile secretory function has not been assessed in ZR.
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    Down-regulation of the Na+/taurocholate cotransporting polypeptide during pregnancy in the rat
    (ELSEVIER SCIENCE BV, 2003) Arrese, M; Traumer, M; Ananthanarayanan, M; Pizarro, M; Solis, N; Accatino, L; Soroka, C; Boyer, JL; Karpen, SJ; Miquel, JF; Suchy, FJ
    Background: Experimental studies have shown decreased bile acid (BA) uptake and reduced excretion of cholephilic compounds in pregnant rodents.
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    The rat liver Na+/bile acid cotransporter - Importance of the cytoplasmic tail to function and plasma membrane targeting
    (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2001) Sun, AQ; Arrese, MA; Zeng, L; Swaby, I; Zhou, MM; Suchy, FJ
    To understand the potential functions of the cytoplasmic tail of Na+/taurocholate cotransporter (Ntcp) and to determine the basolateral sorting mechanisms for this transporter, green fluorescent protein-fused wild type and mutant rat Ntcps were constructed and the transport properties and cellular localization were assessed in transfected COS 7 and Madin-Darby canine kidney (MDCK) cells. Truncation of the 56-amino acid cytoplasmic tail demonstrates that the cytoplasmic tail of rat Ntcp is involved membrane delivery of this protein in nonpolarized and polarized cells and removal of the tail does not affect the bile acid transport function of Ntcp, Using site-directed mutagenesis, two tyrosine residues, Tyr-321 and Tyr-307, in the cytoplasmic tail of Ntcp have been identified as important for the basolateral sorting of rat Ntcp in transfected MDCK cells. Tyr-321 appears to be the major basolateral-sorting determinant, and Tyr-307 acts as a supporting determinant to ensure delivery of the transporter to the basolateral surface, especially at high levels of protein expression, When the two Tyr-based basolateral sorting motifs have been removed, the N-linked carbohydrate groups direct the tyrosine to alanine mutants to the apical surface of transfected MDCK cells. The major basolateral sorting determinant Tyr-321 is within a novel beta -turn unfavorable tetrapeptide Y(321)KAA, which has not been found in any naturally occurring basolateral sorting motifs, Two-dimensional NMR spectroscopy of a 24-mer peptide corresponding to the sequence from Tyr-307 to Thr-330 on the cytoplasmic tail of Ntcp confirms that both the Tyr-321 and Tyr-307 regions do not adopt any turn structure. Since the major motif YKAA contains a beta -turn unfavorable structure, the Ntcp basolateral sorting may not be related to the clathrin-adaptor complex pathway, as is the case for many basolateral proteins.