Browsing by Author "Serrano Honeyman, Carolina"

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    Downregulated Th17 responses are associated with reduced gastritis in Helicobacter pylori-infected children
    (2013) Serrano Honeyman, Carolina; Venegas Muñoz, Alejandro Andrés; Harris D., Paul R.; Serrano Honeyman, Carolina; Venegas Muñoz, Alejandro Andrés; Harris D., Paul R.
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    Early origins of allergy and asthma (ARIES): study protocol for a prospective prenatal birth cohort in Chile.
    (2020) Hernández Vargas, Caroll Daffner; Casanello Toledo, Paola Cecilia; Harris D., Paul R.; Castro Rodríguez, José Antonio; Iturriaga, Carolina; Pérez Mateluna, Guillermo; Farías Jofré, Marcelo Enrique; Urzúa, Marcela; Hernández Carreño, Cherie Francisca; Serrano Honeyman, Carolina; Hernández Vargas, Caroll Daffner; Casanello Toledo, Paola Cecilia; Harris D., Paul R.; Castro Rodríguez, José Antonio; Iturriaga, Carolina; Pérez Mateluna, Guillermo; Farías Jofré, Marcelo Enrique; Urzúa, Marcela; Hernández Carreño, Cherie Francisca; Serrano Honeyman, Carolina
    Abstract Background Growing evidence shows that atopic dermatitis (AD), food allergy (FA), allergic rhinitis, and asthma are largely determined during the first 1000 days (time elapsed from conception to the 2nd birthday). The ARIES birth cohort aims to determine prenatal and perinatal conditions, as well as genetic and epigenetic factors, that participate in the early setting of immune responses, and the role of these in the later determination of the risk of allergic diseases and asthma in the offspring. Methods We have designed a birth cohort of 250 families with prenatal recruitment (~ 14 weeks). We will genotype relevant allergy/asthma-associated variants in trios and will perform immunophenotyping and evaluation of allergy biomarkers in cord blood. At 1 and 2 years of age we will assess if infants have developed allergic sensitization, AD, FA, as well as biomarkers of asthma including the asthma predictive index. We will also evaluate how maternal conditions modify immune programming through epigenetic modifications and will then depict newborn epigenetic cues of allergy/asthma risk. Next, we will assess composition/diversity of maternal gut, placenta, breastmilk and infant gut microbiome and their association with immunophenotype and biomarkers at birth, and clinical outcomes at age 1 and 2. Finally, we plan to assess how environmental exposures (perinatal outdoor and indoor pollution, allergens and endotoxin) affect the incidence of allergic sensitization, AD, FA, and risk of asthma. Discussion The in-depth study of the ARIES birth cohort shall provide crucial information to understand the rising incidence of allergies and asthma in developing countries, and hopefully provide cues on how to prevent and treat these diseases. Trial registration clinicaltrials.gov NCT04186949, retrospectively registered on December 5, 2019.
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    Eradication of Helicobacter pylori in Children Restores the Structure of the Gastric Bacterial Community to That of Noninfected Children
    (2019) Serrano Honeyman, Carolina; Pierre, Reinaldo; Van Der Pol, William J.; Morrow, Casey D.; Smith, Phillip D.; Harris D., Paul R.; Serrano Honeyman, Carolina; Pierre, Reinaldo; Van Der Pol, William J.; Morrow, Casey D.; Smith, Phillip D.; Harris D., Paul R.
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    Functional gastrointestinal disorders in children from low socio-economic status and Helicobacter pylori infection
    (2018) Jaime Méndez, María Francisca; Villagrán Torres, Andrea Alejandra; Hernández Rocha, Cristián Antonio; Ortiz Aparicio, Freddy Miguel; Serrano Honeyman, Carolina; Harris Diez, Paul Richard
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    Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana
    (2008) Serrano Honeyman, Carolina; González Cruz, Javiera; Jauregui, Francisca; Medina, Consuelo; Mancilla Oyarzo, Pablo Eduardo; Matus Picero, José Tomás; Arce Johnson, Jorge Patricio
    Abstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.Abstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.
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    Helicobacter pylori infection and UBT-13C values are associated with changes in body mass index in children and adults
    (2022) Bruera, María J.; Amezquita García, María Virginia; Riquelme Pérez, Arnoldo; Serrano Honeyman, Carolina; Harris D., Paul R.
    Background: The urea breath test (UBT-13C) is a non-invasive technique that allows the diagnosis and confirmation of eradication of Helicobacter pylori infection. Aim: To evaluate H. pylori positivity and values of UBT-13C among infected Chilean children and adults, and to analyze its variation in relation to sex, nutritional status, and age of the patients. Material and Methods: Retrospective study of 1141 patients aged 6 to 94 years, with an indication for a UBT-13C either for diagnosis or for confirmation of eradication of H. pylori infection. 13C enrichment was measured using an infrared spectrometer calculating the delta 13C values before and after the ingestion of 13C marked urea. The clinical data of the patients were obtained at the time of the examination. Results: We included 241 children and 900 adults. Infected children obtained lower UBT-13C delta values than infected adults (16.1 ± 8.7 and 37 ± 52.9, respectively). The rates of infection were higher in males who were recruited for diagnosis. Significant differences were obtained between positivity for H. pylori in overweight and obese children but not adults. UBT-13C titers were significantly associated with the body mass index (BMI) only in adults. Conclusions: H. pylori infection rates are similar between sexes and are higher in children probably because of selection bias. In children, H. pylori positivity is associated with higher BMI and excess malnutrition although with similar UBT-13C values. In adults, H. pylori infection is not related with BMI, but a higher BMI impacts UBT-13C titers.
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    Helicobacter pylori Infection Is Associated with Decreased Expression of SLC5A8, a Cancer Suppressor Gene, in Young Children
    (2016) Orellana Manzano, Andrea; O’Ryan, Miguel G.; Lagomarcino, Anne J.; George, Sergio; Muñoz, Mindy S.; Mamani, Nora; Serrano Honeyman, Carolina; Harris D., Paul R.; Ramilo, Octavio; Mejías, Asunción; Torres, Juan P.; Lucero, Yalda; Quest, Andrew F. G.; Orellana Manzano, Andrea; O’Ryan, Miguel G.; Lagomarcino, Anne J.; George, Sergio; Muñoz, Mindy S.; Mamani, Nora; Serrano Honeyman, Carolina; Harris D., Paul R.; Ramilo, Octavio; Mejías, Asunción; Torres, Juan P.; Lucero, Yalda; Quest, Andrew F. G.
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    Helicobacter pylori pediatric infection changes Fc epsilon RI expression in dendritic cells and Treg profile in vivo and in vitro
    (2019) Leon, M.A.; Palma, C.; Hernandez, C.; Sandoval, M.; Cofre, C.; Pérez Mateluna, G.; Borzutzky Schachter, Arturo; Harris D., Paul R.; Serrano Honeyman, Carolina
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    Helicobacter pylori-associated hypochlorhydria in children, and development of iron deficiency
    (2013) Harris D., Paul R.; Serrano Honeyman, Carolina; Villagrán Torres, Andrea Alejandra; Walker, Marjorie M.; Thomson, Melanie; Duarte, Ignacio; Windle, Henry J.; Crabtree, Jean E.
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    Helicobacter pylori-Clarithromycin Resistance in Symptomatic Pediatric Patients in a High Prevalence Country
    (2017) Serrano Honeyman, Carolina; Leon, Miguel A.; Palma, Camila; Vera, Macarena; Hernandez, Caroll; Harris D., Paul R.; Serrano Honeyman, Carolina; Leon, Miguel A.; Palma, Camila; Vera, Macarena; Hernandez, Caroll; Harris D., Paul R.
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    Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover
    (2020) Alexander, K. L.; Serrano Honeyman, Carolina; Chakraborty, A.; Nearing, M.; Council, L. N.; Riquelme Pérez, Arnoldo; Garrido, M.; Bellis, S. L.; Smythies, L. E.; Smith, P. D.
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    Peptic Ulcer Disease in Helicobacter pylori-Infected Children : Clinical Findings and Mucosal Immune Response
    (2014) Hernández C.; Serrano Honeyman, Carolina; Einisman, H.; Villagrán, A.; Peña Villegas, Alfredo Javier; Duarte, Ignacio; Torres Montes, Paula Javiera; Riera Cassorla, Francisca Paz; Harris D., Paul R.; Hernández C.; Serrano Honeyman, Carolina; Einisman, H.; Villagrán, A.; Peña Villegas, Alfredo Javier; Duarte, Ignacio; Torres Montes, Paula Javiera; Riera Cassorla, Francisca Paz; Harris D., Paul R.
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    Prevalencia de la infección por Helicobacter pylori en niños : estimando la edad de adquisición
    (2013) Jaime Méndez, María Francisca; Villagrán, Andrea; Serrano Honeyman, Carolina; Cerda, Jaime; Harris D., Paul R.; Jaime Méndez, María Francisca; Villagrán, Andrea; Serrano Honeyman, Carolina; Cerda, Jaime; Harris D., Paul R.
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    Rol de la microbiota del jugo gástrico en la respuesta inmune innata de mucosa en la infección pediátrica por h. pylori
    (2019) Hernández Vargas, Caroll Daffner; Hernández Vargas, Caroll Daffner; Serrano Honeyman, Carolina; Harris D., Paul R.; Serrano Honeyman, Carolina; Harris D., Paul R.; Pontificia Universidad Católica de Chile. Facultad de Medicina
    Helicobacter pylori (H. pylori) es una bacteria colonizadora del estómago humano, cuya adquisición temprana en la niñez, está asociada con una modificación en la composición de la microbiota del estómago. Esta tesis estudió el rol de la microbiota del jugo gástrico de niños infectados y no infectados con H. pylori sobre la estructura y función inmune innata que ocurre a nivel de la barrera de células epiteliales gástricas, planteando como hipótesis que la microbiota del jugo gástrico de niños infectados con H. pylori, altera la integridad de la barrera y la respuesta inmune de las células epiteliales gástricas. Se utilizaron cultivos in vitro de la línea celular gástrica AGS y un cultivo primario de monocapas de células epiteliales diferenciadas de stem cells de organoides gástricos pediátricos, los cuales fueron estimulados con el jugo gástrico de niños infectados y no infectados con H. pylori. En estos dos modelos se evaluó los posibles sitios de acción de la microbiota, como los receptores tipo TLRs, proteínas formadoras de uniones estrechas y el efecto sobre factores inmunes como citoquinas y β-defensinas. Adicionalmente, en el cultivo de monocapa de células epiteliales diferenciadas de organoides, se evaluó distintos patrones de distribución de la proteína formadora de unión estrecha zónulaocludens 1 (ZO-1), que incluyeron quiebres en la estructura de la barrera de células epiteliales, inmuno-tinción de ZO-1 difusa, débil, puntiforme y formación de estructuras en forma de anillos. También, en este modelo se estudió la primo infección por H. pylori por medio de un ensayo de desafío con la bacteria en células pre-incubadas con la microbiota del jugo gástrico de niños no infectados, evaluando el efecto sobre los patrones de distribución de ZO-1. En el modelo de células AGS, se encontró que el jugo gástrico de niños no infectados indujo una disminución de la expresión de mRNA de claudina-2 (Cldn-2) y de la βdefensina-1 (hBD-1) en comparación a las células sin tratar. Por otro lado, el jugo gástrico de niños infectados con H. pylori produjo un aumento de la expresión de mRNA del receptor TLR-2. Tanto en células AGS como en monocapa de células epiteliales el jugo gástrico ejerció un aumento en la secreción de IL-8 y TNF-α de manera dependiente de la presencia del componente bacteriano e independiente del estatus de infección por H. pylori. En las monocapas de células epiteliales, la microbiota del jugo gástrico de ambos grupos, indujo la expresión de la β-defensina-2 (hBD-2), mientras que el jugo gástrico de niños infectados con H. pylori indujo la expresión de IL-8 y TNF-α y un aumento en el porcentaje de células que presentan estructuras en forma de anillo de ZO-1, lo cual es dependiente de la presencia del componente bacteriano. El análisis de las estructuras en forma de anillo de ZO-1, sugirió la formación de un poro entre la unión célula-célula, lo cual podría modificar la función de la barrera de células epiteliales. Finalmente, el ensayo de primo-infección por H. pylori mostró que el jugo gástrico de niños no infectados disminuye la alteración estructural de la barrera de célula epiteliales producida por infección temprana de H. pylori. En conclusión, la microbiota del jugo gástrico de niños sanos previene el efecto inducido por la infección temprana por H. pylori, estimulando la respuesta inmune innata y disminuyendo la alteración estructural de la barrera de célula epiteliales. Sin embargo, si los mecanismos de defensa son sobrepasados y se establece la infección por H. pylori, la microbiota del jugo gástrico asociada a la infección estimula la respuesta inmune innata para hacerla más efectiva, pero altera la integridad de la barrera de las células epiteliales gástricas, lo que podría tener un rol en el daño asociado a la infección. De este modo, el jugo gástrico de niños no infectados por H. pylori, podría ser un posible blanco terapéutico para modular la respuesta inmune y disminuir alteraciones en la estructura de la barrera en las células epiteliales gástricas en presencia de H. pylori, sin la necesidad de erradicar la bacteria de su nicho.
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    Serum periostin is not related to asthma predictive index
    (2018) Castro Rodríguez, José Antonio; Atton, I.; Villarroel S, G.; Serrano Honeyman, Carolina