Browsing by Author "Schiavo, Giampietro"
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- ItemAn Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons(2020) Stuardo, Nicolas; Moya-Alvarado, Guillermo; Ramirez, Carolina; Schiavo, Giampietro; Bronfman, Francisca C.Recombinant BDNF containing an Avi sequence (BDNFAvi) is produced in HEK293 cells and then cost-effectively purified by affinity chromatography. A reproducible protocol was developed to directly mono-biotinylate BDNFAvi with the enzyme BirA in a tube. In this reaction, mono-biotinylated BDNFAvi retains its biological activity.