Browsing by Author "Sandoval, M."

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    CHRONIC FLUOXETINE TREATMENT INDUCES STRUCTURAL PLASTICITY AND SELECTIVE CHANGES IN GLUTAMATE RECEPTOR SUBUNITS IN THE RAT CEREBRAL CORTEX
    (PERGAMON-ELSEVIER SCIENCE LTD, 2010) Ampuero, E.; Rubio, F. J.; Falcon, R.; Sandoval, M.; Diaz Veliz, G.; Gonzalez, R. E.; Earle, N.; Dagnino Subiabre, A.; Aboitiz, F.; Orrego, F.; Wyneken, U.
    It has been postulated that chronic administration of antidepressant drugs induces delayed structural and molecular adaptations at glutamatergic forebrain synapses that might underlie mood improvement. To gain further insight into these changes in the cerebral cortex, rats were treated with fluoxetine (flx) for 4 weeks. These animals showed decreased anxiety and learned helplessness. N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunit levels (NR1, NR2A, NR2B, GluR1 and GluR2) were analysed in the forebrain by both western blot of homogenates and immunohistochemistry. Both methods demonstrated an upregulation of NR2A, GluR1 and GluR2 that was especially significant in the retrosplenial granular b cortex (RSGb). However, when analysing subunit content in postsynaptic densities and synaptic membranes, we found increases of NR2A and GluR2 but not GluR1. Instead, GluR1 was augmented in a microsomal fraction containing intracellular membranes. NR1 and GluR2 were co-immunoprecipitated from postsynaptic densities and synaptic membranes. In the immunoprecipitates, NR2A was increased while GluR1 was decreased supporting a change in receptor stoichiometry. The changes of subunit levels were associated with an upregulation of dendritic spine density and of large, mushroom-type spines. These molecular and structural adaptations might be involved in neuronal network stabilization following long-term fix treatment. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
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    Cutaneous Manifestations in Renal Transplant Recipients of Santiago, Chile
    (ELSEVIER SCIENCE INC, 2009) Sandoval, M.; Ortiz, M.; Diaz, C.; Majerson, D.; Molgo, M.
    Introduction. Renal transplant recipients have a heightened risk of developing various cutaneous manifestations, such as skin infections, skin cancer, and secondary effects of immunosuppressive drugs. These manifestations differ depending on the evaluated population. The objective of this study was to describe the prevalence of cutaneous manifestations among renal transplant recipients in Chile between 1979 and 2008.
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    Helicobacter pylori pediatric infection changes Fc epsilon RI expression in dendritic cells and Treg profile in vivo and in vitro
    (2019) Leon, M.A.; Palma, C.; Hernandez, C.; Sandoval, M.; Cofre, C.; Pérez Mateluna, G.; Borzutzky Schachter, Arturo; Harris D., Paul R.; Serrano Honeyman, Carolina
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    Latin American Registry of Pediatric Renal Transplantation 2004-2008
    (2010) Goulart, P.; Koch, P.; Medina-Pestana, J.; Garcia, C.; Bittencourt, V.; Medeiros, M.; Munoz, R.; Delucchi, A.; Lillo, A. M.; Ariza, M.; Bosque, M.; Carvalho, D.; Matuck, T.; Meneses, R.; Fontes, J.; Monteiro, D. C.; Neto, E. Davi; Pinto, V.; Salas, P.; Prates, L.; Belanguero, V.; Pereira, L.; Lima, E.; Penido, J. M.; Benini, V.; Laranjo, S.; Silva, J. M.; Orta, N.; Coronel, V.; Cisneros, A.; Arriaga, J.; Sebastian, M. J.; Abbud-Filho, M.; Fernandez, I.; Gastelbondo, R.; Medjia, N.; Rosati, P.; Hevia, P.; Baptista, M. A.; Ramalho, H.; Diaz, M.; Monteverde, M.; Ferraris, J.; Repetto, H.; Exeni, R.; Florentin, L.; Florin, J.; Cazorla, N.; Casadei, D.; Melendez, K.; Calderon, R.; Silva, V.; Patino, J.; Palacio, D.; Madrigal, G.; Sandoval, M.; Urbina, C.; Loza, R.; Cavagnaro, F.; Vogel, A.; Jimenez, W.; Lou, R.; Rodriguez, C.; Aguilar, C.; Galvez, H.; Rodriguez, L.; Paladini, J.; Agusti, J.; Puelma, F.; Troche, A.; Martinez-Pico, M.; Higueras, W.; Liendo, C.; Restrepo, J.; Caicedo, L. A.; Socorro, F.; Semprum, P.
    The Latin American Pediatric Nephrology Association (ALANEPE) reports the first regional kidney transplant registry in Latin America. A mean range of 75%, 30% to 100% renal transplant recipients under 21 yr old were included. Out of 20 countries invited to participate, 14 performed renal transplant, 11 had universal financial support from their governments. The centers included: Brazil (9), Argentina (4), Chile (4), Venezuela (3), Mexico (2), and one in: Cuba, Colombia, Costa Rica, Nicaragua, Guatemala, Ecuador, Honduras, Paraguay and Peru. The registry included 1458 patients, average of 291 per year, 55% male. Mean follow-up 23.4 +/- 17 months; mean age was 11.7 +/- 4.3 yr (1-21), 11% under five yr of age. Etiology: uropathy/ reflux nephropathy 27%, glomerulopathies 24% (included 12% FSGS), hypo/dysplasia (11%), vascular (6%), congenital/hereditary (5%), unknown (19%). Induction therapy: 71% anti-IL2RAb, 13% ATG/TIMO, 14% non-induction. Maintenance therapy: Tacrolimus 64%, Cyclosporine 32%, MMF 54%, MPS 20%, noTORi 96%, steroids 90%, withdrawal or steroid avoidance 10%. Loss of graft 155/1458 (11%), death with functioning graft (3.4%), vascular thrombosis (2.8%), acute rejection (2.8%), recurrence of disease (1%). Forty-eight patients died (3.3%); infection was the main cause 23 (2.1%). Global patient survival rate at one, three, and five yr was 97%, 96%, and 96%. Graft survival rate at one, three, and five yr LRD was 96%, 93% and 89%; for DD 92%, 86% and 76% respectively. Both survival rates were higher in LRD (p < 0.008 and p < 0.001). A pediatric renal transplant study has started, making information available to be shared between the centers and the world.