Browsing by Author "Salinas Grenet, Hernán"

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    Enhanced auxin signaling promotes root-hair growth at moderately low temperature in Arabidopsis thaliana
    (Cell Press, 2025) Berdion Gabarain, Victoria; Nuñez Lillo, Gerardo; Pencik, Ales; Carignani Sardoy, Mariana; Ibeas, Miguel Angel; Salinas Grenet, Hernán; Martínez Pacheco, Javier; López, Leonel; Rossi, Andrés Hugo; Miglietta, Esteban A.; Guidobono, Juan Santiago; Zhongtao, Jia; Novak, Ondrej; Hettwer Giehl, Ricardo Fabiano; Wiren, Nicolaus von; Meneses Araya, Claudio Antonio; Estévez, José Manuel
    Root hairs (RHs) are mixed tip- and non-tip-growing protrusions derived from root epidermal cells that play essential roles in nutrient and water uptake, root anchorage, and interactions with soil microorganisms. Nutrient availability and temperature are critical and interconnected factors for sustained plant growth, but the molecular mechanisms that underlie their perception and downstream signaling pathways remain unclear. Here, we show that moderately low temperature (10°C) induces a strong RH elongation response mediated by several molecular components of the auxin pathway. Specifically, auxin biosynthesis mediated by TAA1/YUCCAs, auxin transport via PIN2, PIN4, and AUX1, and auxin signaling regulated by TIR1/AFB2 in conjunction with specific ARFs (ARF6/ARF8 and ARF7, but not ARF19) contribute to the RH response under moderately low temperature. These findings establish the auxin biosynthesis and signaling pathway as a central regulatory process driving RH growth under moderate low-temperature conditions in roots. Our work underscores the importance of moderately low temperature as a stimulus that interacts with complex nutritional signaling originating from the growth medium and the plant nutritional status; this process has the potential to be fine-tuned for future biotechnological applications to enhance nutrient uptake.
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    Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature linked to a low-nutrient environment
    (WILEY, 2025) Urzúa Lehuede, Tomás; Berdion Gabarain, Victoria; Ibeas, Miguel Angel; Salinas Grenet, Hernán; Achá Escobar, Romina; Moyano, Tomás C.; Ferrero, Lucía; Núñez Lillo, Gerardo; Pérez Díaz, Jorge; Perotti, María Florencia; Natali Miguel, Virginia; Spies, Fiorella Paola; Rosas, Miguel A.; Kawamura, Ayako; Rodríguez García, Diana R.; Kim, Ah-Ram; Nolan, Trevor; Moreno, Adrian A.; Sugimoto, Keiko; Perrimon, Norbert; Sanguinet, Karen A.; Meneses Araya, Claudio Antonio; Chan, Raquel L.; Ariel, Federico; Alvárez, José M.; Estévez, José M.
    Root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous and external signals, which are combined to form an integrative response. Surprisingly, a low-temperature condition of 10 degrees C causes increased RH growth in Arabidopsis and in several monocots, even when the development of the rest of the plant is halted. Previously, we demonstrated a strong correlation between RH growth response and a significant decrease in nutrient availability in the growth medium under low-temperature conditions. However, the molecular basis responsible for receiving and transmitting signals related to the availability of nutrients in the soil, and their relation to plant development, remain largely unknown. We have discovered two antagonic gene regulatory networks (GRNs) controlling RH early transcriptome responses to low temperature. One GNR enhances RH growth and it is commanded by the transcription factors (TFs) ROOT HAIR DEFECTIVE 6 (RHD6), HAIR DEFECTIVE 6-LIKE 2 and 4 (RSL2-RSL4) and a member of the homeodomain leucine zipper (HD-Zip I) group I 16 (AtHB16). On the other hand, a second GRN was identified as a negative regulator of RH growth at low temperature and it is composed by the trihelix TF GT2-LIKE1 (GTL1) and the associated DF1, a previously unidentified MYB-like TF (AT2G01060) and several members of HD-Zip I group (AtHB3, AtHB13, AtHB20, AtHB23). Functional analysis of both GRNs highlights a complex regulation of RH growth response to low temperature, and more importantly, these discoveries enhance our comprehension of how plants synchronize RH growth in response to variations in temperature at the cellular level.