Browsing by Author "SPENCER, E"

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    RESPIRATORY SYNCYTIAL VIRUS DETECTION BY DOT BLOT HYBRIDIZATION WITH A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE
    (1992) HERNANDEZ, O; FERNANDEZ, J; VALENZUELA, S; SANDINO, AM; PIZARRO, J; VASQUEZ, M; YUDELEVICH, A; SPENCER, E
    A synthetic oligodeoxynucleotide corresponding to a region of the nucleocapside gene (N) of respiratory syncytial virus (RSV), was used as a DNA probe to develop a nonradioactive hybridization assay for the detection of RSV. The probe was labeled by incorporation of biotin-7-dATP to the 3' end by a reaction catalyzed by terminal deoxynucleotydil transferase.
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    ROTAVIRUS DETECTION BY DOT BLOT HYBRIDIZATION ASSAY USING A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE
    (1992) FERNANDEZ, J; SANDINO, A; YUDELEVICH, A; AVENDANO, LF; VENEGAS, A; HINRICHSEN, V; SPENCER, E
    A synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus. was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.