Browsing by Author "SANDINO, AM"
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- ItemRESPIRATORY SYNCYTIAL VIRUS DETECTION BY DOT BLOT HYBRIDIZATION WITH A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE(1992) HERNANDEZ, O; FERNANDEZ, J; VALENZUELA, S; SANDINO, AM; PIZARRO, J; VASQUEZ, M; YUDELEVICH, A; SPENCER, EA synthetic oligodeoxynucleotide corresponding to a region of the nucleocapside gene (N) of respiratory syncytial virus (RSV), was used as a DNA probe to develop a nonradioactive hybridization assay for the detection of RSV. The probe was labeled by incorporation of biotin-7-dATP to the 3' end by a reaction catalyzed by terminal deoxynucleotydil transferase.