Browsing by Author "Rojas, Barbara"

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    Metabolomic and biochemical analysis of mesocarp tissues from table grape berries with contrasting firmness reveals cell wall modifications associated to harvest and cold storage
    (2022) Balic, Ivan; Olmedo, Patricio; Zepeda, Baltasar; Rojas, Barbara; Ejsmentewicz, Troy; Barros, Miriam; Aguayo, Daniel; Moreno, Adrian A.; Pedreschi, Romina; Meneses, Claudio; Campos-Vargas, Reinaldo
    Tissue texture influences the grape berry consumers acceptance. We studied the biological differences between the inner and outer mesocarp tissues in hard and soft berries of table grapes cv NN107. Texture analysis revealed lower levels of firmness in the inner mesocarp as compared with the outer tissue. HPAEC-PAD analysis showed an increased abundance of cell wall monosaccharides in the inner mesocarp of harder berries at harvest. Immunohistochemical analysis displayed differences in homogalacturonan methylesterification and cell wall calcium between soft and hard berries. This last finding correlated with a differential abundance of calcium measured in the alcohol-insoluble residues (AIR) of the inner tissue of the hard berries. Analysis of abundance of polar metabolites suggested changes in cell wall carbon supply precursors, providing new clues in the identification of the biochemical factors that define the texture of the mesocarp of grape berries.
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    Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries
    (2023) Olmedo, Patricio; Nunez-Lillo, Gerardo; Vidal, Juan; Leiva, Carol; Rojas, Barbara; Sagredo, Karen; Arriagada, Cesar; Defilippi, Bruno G.; Perez-Donoso, Alonso G.; Meneses, Claudio; Carpentier, Sebastien; Pedreschi, Romina; Campos-Vargas, Reinaldo
    Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N- (2-chloro-4-pyridinyl)-N '-phenylurea (CPPU) is widely used, its effect on grape quality is poorly understood. We hypothesized that the use of CPPU in pre-flowering can lead to changes in the metabolism that affects grape quality at harvest. Therefore, we investigated the role of CPPU applications on the quality of grapes by inte-grating proteomics and metabolomics. CPPU-treated grapevines showed a significant increase in berry size and firmness. Proteomic analyses indicated that CPPU-treated berries accumulated enzymes associated with carbo-hydrate metabolism, glycolysis, and tricarboxylic acid (TCA) cycle at harvest. Metabolomic analyses showed shifts in the abundance of compounds associated with carbohydrate metabolism and TCA cycle in CPPU-treated grapes. These findings suggest that CPPU applications modulate central carbon metabolism, improving grape berry quality.